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Literature summary for 2.7.7.1 extracted from

  • Emanuelli, M.; Natalini, P.; Raffaelli, N.; Ruggieri, S.; Vita, A.; Magni, G.
    NAD biosynthesis in human placenta: purification and characterization of homogeneous NMN adenylyltransferase (1992), Arch. Biochem. Biophys., 298, 29-34.
    View publication on PubMed

General Stability

General Stability Organism
dithiothreitol and EDTA stabilize during purification Homo sapiens
extremely labile in crude extracts, even in the presence of PMSF and sodium metabisulfite, EDTA or DTT does not stabilize Homo sapiens
very sensitive to freeze-thawing, 50% glycerol stabilizes Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
ADPribose reverse reaction Homo sapiens
beta-NMNH
-
Homo sapiens
Cd2+
-
Homo sapiens
Cr3+
-
Homo sapiens
Cu2+ strong Homo sapiens
Fe3+
-
Homo sapiens
Hg2+
-
Homo sapiens
additional information no inhibition by pyrimidine or purine bases, oxy- and deoxynucleosides, cAMP, halogenated and methylated nucleobases, oxy- and deoxyribose, oxy- and deoxyribose 5-phosphate, D-alpha-glucose, D-alpha-glucose 1-phosphate, D-alpha-glucose 6-phosphate, D-alpha-glucose 1,6-diphosphate, D-alpha-fructose, D-alpha-fructose 1,6-diphosphate, alpha-nicotinamide ribonucleotide; product inhibition study Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.023
-
ATP 37°C, pH 7.6 Homo sapiens
0.038
-
nicotinamide ribonucleotide 37°C, pH 7.6 Homo sapiens
0.067 0.069 NAD+ 37°C, pH 7.6 Homo sapiens
0.125
-
diphosphate 37°C, pH 7.6 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus
-
Homo sapiens 5634
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ requirement Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
alpha4, 4 * 33000, SDS-PAGE, with or without 2-mercaptoethanol Homo sapiens
132000
-
gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + nicotinamide ribonucleotide Homo sapiens key reaction of pyridine nucleotide metabolism diphosphate + NAD+
-
r

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
ATP + nicotinamide ribonucleotide = diphosphate + NAD+ mechanism Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
placenta
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
9.1 10.2
-
Homo sapiens

Storage Stability

Storage Stability Organism
-80°C, 1 mM DTT, 0.5 mM EDTA, 2 weeks Homo sapiens
4°C, in 50 mM potassium phosphate buffer, pH 7.4, 1 mM DTT, 1 mM KCl, 1 mM MgCl2, 0.5 mM EDTA, 1 mM PMSF, several months Homo sapiens
high KCl concentrations stabilize during storage Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + nicotinamide ribonucleotide best substrate Homo sapiens diphosphate + NAD+
-
r
ATP + nicotinamide ribonucleotide strongly stereospecific: beta-, not alpha-anomer of nicotinamide ribonucleotide Homo sapiens diphosphate + NAD+
-
r
ATP + nicotinamide ribonucleotide i.e. NMN or nicotinamide mononucleotide Homo sapiens diphosphate + NAD+
-
r
ATP + nicotinamide ribonucleotide key reaction of pyridine nucleotide metabolism Homo sapiens diphosphate + NAD+
-
r
ATP + nicotinate ribonucleotide i.e. nicotinate mononucleotide Homo sapiens diphosphate + nicotinic acid-adenine dinucleotide
-
r
ATP + nicotinate ribonucleotide less efficient Homo sapiens diphosphate + nicotinic acid-adenine dinucleotide
-
r

Subunits

Subunits Comment Organism
tetramer alpha4, 4 * 33000, SDS-PAGE, with or without 2-mercaptoethanol Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 9 plateau Homo sapiens

pI Value

Organism Comment pI Value Maximum pI Value
Homo sapiens isoelectric focusing, LKB 8100 ampholine column filled with a linear gradient of glycerol containing 1% ampholine carrier ampholytes 4.8 4.7