General Stability | Organism |
---|---|
dithiothreitol and EDTA stabilize during purification | Homo sapiens |
extremely labile in crude extracts, even in the presence of PMSF and sodium metabisulfite, EDTA or DTT does not stabilize | Homo sapiens |
very sensitive to freeze-thawing, 50% glycerol stabilizes | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADPribose | reverse reaction | Homo sapiens | |
beta-NMNH | - |
Homo sapiens | |
Cd2+ | - |
Homo sapiens | |
Cr3+ | - |
Homo sapiens | |
Cu2+ | strong | Homo sapiens | |
Fe3+ | - |
Homo sapiens | |
Hg2+ | - |
Homo sapiens | |
additional information | no inhibition by pyrimidine or purine bases, oxy- and deoxynucleosides, cAMP, halogenated and methylated nucleobases, oxy- and deoxyribose, oxy- and deoxyribose 5-phosphate, D-alpha-glucose, D-alpha-glucose 1-phosphate, D-alpha-glucose 6-phosphate, D-alpha-glucose 1,6-diphosphate, D-alpha-fructose, D-alpha-fructose 1,6-diphosphate, alpha-nicotinamide ribonucleotide; product inhibition study | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.023 | - |
ATP | 37°C, pH 7.6 | Homo sapiens | |
0.038 | - |
nicotinamide ribonucleotide | 37°C, pH 7.6 | Homo sapiens | |
0.067 | 0.069 | NAD+ | 37°C, pH 7.6 | Homo sapiens | |
0.125 | - |
diphosphate | 37°C, pH 7.6 | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
nucleus | - |
Homo sapiens | 5634 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | requirement | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
alpha4, 4 * 33000, SDS-PAGE, with or without 2-mercaptoethanol | Homo sapiens |
132000 | - |
gel filtration | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + nicotinamide ribonucleotide | Homo sapiens | key reaction of pyridine nucleotide metabolism | diphosphate + NAD+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + nicotinamide ribonucleotide = diphosphate + NAD+ | mechanism | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
placenta | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
9.1 | 10.2 | - |
Homo sapiens |
Storage Stability | Organism |
---|---|
-80°C, 1 mM DTT, 0.5 mM EDTA, 2 weeks | Homo sapiens |
4°C, in 50 mM potassium phosphate buffer, pH 7.4, 1 mM DTT, 1 mM KCl, 1 mM MgCl2, 0.5 mM EDTA, 1 mM PMSF, several months | Homo sapiens |
high KCl concentrations stabilize during storage | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + nicotinamide ribonucleotide | best substrate | Homo sapiens | diphosphate + NAD+ | - |
r | |
ATP + nicotinamide ribonucleotide | strongly stereospecific: beta-, not alpha-anomer of nicotinamide ribonucleotide | Homo sapiens | diphosphate + NAD+ | - |
r | |
ATP + nicotinamide ribonucleotide | i.e. NMN or nicotinamide mononucleotide | Homo sapiens | diphosphate + NAD+ | - |
r | |
ATP + nicotinamide ribonucleotide | key reaction of pyridine nucleotide metabolism | Homo sapiens | diphosphate + NAD+ | - |
r | |
ATP + nicotinate ribonucleotide | i.e. nicotinate mononucleotide | Homo sapiens | diphosphate + nicotinic acid-adenine dinucleotide | - |
r | |
ATP + nicotinate ribonucleotide | less efficient | Homo sapiens | diphosphate + nicotinic acid-adenine dinucleotide | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | alpha4, 4 * 33000, SDS-PAGE, with or without 2-mercaptoethanol | Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | plateau | Homo sapiens |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Homo sapiens | isoelectric focusing, LKB 8100 ampholine column filled with a linear gradient of glycerol containing 1% ampholine carrier ampholytes | 4.8 | 4.7 |