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Literature summary for 2.7.6.5 extracted from

  • Wilkinson, R.C.; Batten, L.E.; Wells, N.J.; Oyston, P.C.; Roach, P.L.
    Biochemical studies on Francisella tularensis RelA in (p)ppGpp biosynthesis (2015), Biosci. Rep., 35, e00268.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.6.5

Activating Compound

Activating Compound Comment Organism Structure
guanosine-5',3'-dibisphosphate ppGpp, potential allosteric regulator, activates the enzyme with an EC50 of 0.060 mM Francisella tularensis subsp. tularensis
additional information activation of Francisella tularensis RelA by stalled ribosomal complexes formed with ribosomes purified from Escherichia coli MRE600, and significantly weaker activation with ribosomes isolated from Francisella philomiragia Francisella tularensis subsp. tularensis

Cloned(Commentary)

Cloned (Comment) Organism
gene relA, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21 (DE3) pLysS Francisella tularensis subsp. tularensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information steady-state kinetic analysis, saturation activity curves best fit a sigmoidal function, the kinetic profile can result from allosteric regulation, overview Francisella tularensis subsp. tularensis
0.259 0.344 ATP pH 8.0, 30°C, recombinant enzyme Francisella tularensis subsp. tularensis
0.801 1.024 GTP pH 8.0, 30°C, recombinant enzyme Francisella tularensis subsp. tularensis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Francisella tularensis subsp. tularensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
74000
-
-
 Francisella tularensis subsp. tularensis
128000
-
 recombinant His-tagged enzyme, gel filtration Francisella tularensis subsp. tularensis
148000
-
 about, sequence calculation Francisella tularensis subsp. tularensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + GTP Francisella tularensis subsp. tularensis
-
 AMP + guanosine 3'-diphosphate 5'-triphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Francisella tularensis subsp. tularensis Q5NEV0 gene relA or FTT_1508c
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3) pLysS by nickel affinity chromatography and gel filtration Francisella tularensis subsp. tularensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + GTP
-
 Francisella tularensis subsp. tularensis AMP + guanosine 3'-diphosphate 5'-triphosphate
-
 ?
ATP + GTP high degree of specificity for GTP as a pyrophosphate acceptor, with no measurable turnover for GDP Francisella tularensis subsp. tularensis AMP + guanosine 3'-diphosphate 5'-triphosphate
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 74000, recombinant His-tagged enzyme, SDS-PAGE Francisella tularensis subsp. tularensis

Synonyms

Synonyms Comment Organism
RelA
-
 Francisella tularensis subsp. tularensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Francisella tularensis subsp. tularensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Francisella tularensis subsp. tularensis

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Francisella tularensis subsp. tularensis

General Information

General Information Comment Organism
evolution the enzyme RelA belongs to the RSH superfamily, also comprising members such as RelA/SpoT homologue, (p)ppGpp synthetase I, SpoT, and (p)ppGpp synthetase II. The RSH enzyme family controls concentrations of the alarmones (p)ppGpp (guanosine penta- or tetra-phosphate) Francisella tularensis subsp. tularensis