Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | addition of template, unacylated tRNA and ribosomes to the activity assay stimulates SF 30fold when using optimal conditions, activity of SF increases threefold in the presence of twice salt-washed tight-couple ribosomes and twofold in the presence of reassociated ribosomes compared to the endogenous activity of the enzyme | Escherichia coli | |
unacylated tRNA | in the ribosomal amino-acyl site (A-site) | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21(DE3) | Escherichia coli |
General Stability | Organism |
---|---|
the purified SF is stored in the freezer, at a concentration of 1.0 mg/ml in 20% (v/v) glycerol, without forming a precipitate or loss of activity | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
micrococcin | ribosome-dependent activity | Escherichia coli | |
tetracycline | endogenous diphoyphoryl transferase activity; ribosome-dependent activity | Escherichia coli | |
Thiostrepton | ribosome-dependent activity | Escherichia coli | |
viomycin | ribosome-dependent activity | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | the magnesium concentration also affects the binding states of the tRNAs on the ribosome | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + GTP | Escherichia coli | - |
AMP + guanosine 5'-triphosphate 3'-diphosphate | - |
r | |
ATP + GTP | Escherichia coli MRE 600 | - |
AMP + guanosine 5'-triphosphate 3'-diphosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
MRE 600 | - |
Escherichia coli MRE 600 | - |
MRE 600 | - |
Purification (Comment) | Organism |
---|---|
affinity-chromatography and precipitation | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + GTP | - |
Escherichia coli | AMP + guanosine 5'-triphosphate 3'-diphosphate | - |
r | |
ATP + GTP | - |
Escherichia coli MRE 600 | AMP + guanosine 5'-triphosphate 3'-diphosphate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
ATP:GTP pyrophosphoryl transferase | - |
Escherichia coli |
SF | - |
Escherichia coli |
stringent factor | - |
Escherichia coli |