Application | Comment | Organism |
---|---|---|
drug development | the structural knowledge of the self-regulatory mechanisms for controlling the opposing catalytic activities of RelSeq can be used to design specific inhibitors that interfere with either of the active sites and may have the potential of being developped into powerful antibacterial drugs | Streptococcus dysgalactiae |
Cloned (Comment) | Organism |
---|---|
expression of the N-terminal catalytic fragment (residues 1-385) in Escherichia coli BL21 | Streptococcus dysgalactiae |
Crystallization (Comment) | Organism |
---|---|
the crystallographic asymmetric unit contains two copies of RelSeq 1-385, two catalytic domains are clearly evident within each monomer, with the hydrolase (residues 5-159) and the synthetase (residues 176-371) domains joined by an overlapping central 3-helix bundle (residues 135-195) | Streptococcus dysgalactiae |
Protein Variants | Comment | Organism |
---|---|---|
D264G | eliminates detectable synthetase activity without appreciably altering the hydrolase activity | Streptococcus dysgalactiae |
E323Q | eliminates detectable synthetase activity without appreciably altering the hydrolase activity | Streptococcus dysgalactiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
alpha,beta-methylene-ATP | - |
Streptococcus dysgalactiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | essential for activation, the enzyme has a single Mg2+ binding site | Streptococcus dysgalactiae | |
Mn2+ | necessary cofactor at the hydrolase active site | Streptococcus dysgalactiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + GTP | Streptococcus dysgalactiae | bifunctional enzyme with synthetase and hydrolase activities | AMP + guanosine 5'-triphosphate 3'-diphosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus dysgalactiae | - |
subsp. equisimilis | - |
Purification (Comment) | Organism |
---|---|
the catalytic fragment RelSeq 1-385 | Streptococcus dysgalactiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + GTP | bifunctional enzyme with synthetase and hydrolase activities | Streptococcus dysgalactiae | AMP + guanosine 5'-triphosphate 3'-diphosphate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
Rel/Spo protein | - |
Streptococcus dysgalactiae |
RelSeq | - |
Streptococcus dysgalactiae |