Literature summary for 2.7.6.4 extracted from

Oki, T.; Yoshimoto, A.; Ogasawara, T.; Sato, S.; Takamatsu, A.
Occurrence of pppApp-synthesizing activity in actinomycetes and isolation of purine nucleotide pyrophosphotransferase (1976), Arch. Microbiol., 107, 183-187.

Search for more literature for 2.7.6.4

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.25	-	GDP	pH 10.0, 37°C, reaction with dATP	Streptomyces morookaense
0.44	-	ADP	pH 10.0, 37°C reaction with ATP	Streptomyces aspergilloides
0.46	-	GDP	pH 10.0, 37°C, reaction with ATP	Streptomyces morookaense
0.47	-	GTP	pH 10.0, 37°C, reaction with dATP	Streptomyces morookaense
0.77	-	ADP	pH 10.0, 37°C reaction with ATP	Streptomyces aspergilloides
0.89	-	GMP	pH 10.0, 37°C, reaction with ATP	Streptomyces morookaense
1.61	-	ADP	pH 10.0, 37°C, reaction with pppApp	Streptomyces morookaense
1.67	-	GTP	pH 10.0, 37°C, reaction with pppApp	Streptomyces morookaense
1.75	-	GTP	pH 10.0, 37°C reaction with ATP	Streptomyces morookaense
2.38	-	ADP	pH 10.0, 37°C reaction with ATP	Streptomyces aspergilloides
2.78	-	AMP	pH 10.0, 37°C, reaction with ATP	Streptomyces morookaense
3.23	-	ADP	pH 10.0, 37°C, reaction with ATP	Streptomyces morookaense
16.67	-	ADP	pH 10.0, 37°C reaction with dATP	Streptomyces morookaense

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	-	Streptomyces hachijoensis	-	-
extracellular	-	Streptomyces violascens	-	-
extracellular	-	Streptomyces morookaense	-	-
extracellular	-	Streptomyces aspergilloides	-	-
extracellular	-	Streptomyces septatus	-	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
18000	-	gel filtration	Streptomyces hachijoensis
21000	-	gel filtration	Streptomyces septatus
21000	-	1 * 21000, SDS-PAGE	Streptomyces aspergilloides
23000	-	gel filtration	Streptomyces aspergilloides
24000	-	1 * 24000, SDS-PAGE	Streptomyces morookaense
25000	-	gel filtration	Streptomyces morookaense
26000	-	gel filtration	Streptomyces violascens

Organism

Organism	UniProt	Comment	Textmining
Streptomyces aspergilloides	-	-	-
Streptomyces hachijoensis	-	-	-
Streptomyces morookaense	-	-	-
Streptomyces septatus	-	-	-
Streptomyces violascens	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
culture filtrate	-	Streptomyces morookaense	-
mycelium	weak activity	Streptomyces violascens	-
mycelium	weak activity	Streptomyces morookaense	-
mycelium	weak activity	Streptomyces aspergilloides	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + ADP	-	Streptomyces morookaense	AMP + adenosine 3'-diphosphate 5'-diphosphate	-	?
ATP + ADP	-	Streptomyces aspergilloides	AMP + adenosine 3'-diphosphate 5'-diphosphate	-	?
ATP + AMP	-	Streptomyces morookaense	AMP + adenosine 3'-phosphate 5'-diphosphate	-	?
ATP + GDP	-	Streptomyces morookaense	AMP + guanosine 3'-diphosphate 5'-diphosphate	-	?
ATP + GDP	-	Streptomyces aspergilloides	AMP + guanosine 3'-diphosphate 5'-diphosphate	-	?
ATP + GMP	-	Streptomyces morookaense	AMP + guanosine 3'-phosphate 5'-diphosphate	-	?
ATP + GTP	-	Streptomyces morookaense	AMP + guanosine-3'-diphosphate-5'-triphosphate	-	?
ATP + GTP	-	Streptomyces aspergilloides	AMP + guanosine-3'-diphosphate-5'-triphosphate	-	?
dATP + ADP	-	Streptomyces morookaense	dAMP + adenosine 3'-diphosphate 5'-diphosphate	-	?
dATP + GDP	-	Streptomyces morookaense	dAMP + guanosine 3'-diphosphate 5'-diphosphate	-	?
dATP + GTP	-	Streptomyces morookaense	dAMP + guanosine 3'-diphosphate 5'-triphosphate	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 24000, SDS-PAGE	Streptomyces morookaense
monomer	1 * 18000, SDS-PAGE	Streptomyces hachijoensis
monomer	1 * 21000, SDS-PAGE	Streptomyces aspergilloides

pI Value

Organism	Comment	pI Value Maximum	pI Value
Streptomyces aspergilloides	isoelectric focusing	-	6.85
Streptomyces morookaense	isoelectric focusing	-	6.9
Streptomyces violascens	-	-	7.54
Streptomyces hachijoensis	isoelectric focusing	-	8.3
Streptomyces septatus	isoelectric focusing	-	9.2

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Release 2023.1 (January 2023)