Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Plasmodium falciparum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Guanidine-HCl | 0.25 M, 50% inhibitioin | Plasmodium falciparum | |
KCl | activation at 0.4 M, inhibition at higher concentration | Plasmodium falciparum | |
NaCl | activation at 0.2 M, inhibition at higher concentration | Plasmodium falciparum | |
sulfadoxine | competitive, moderately potent | Plasmodium falciparum | |
sulfathiazole | competitive | Plasmodium falciparum | |
Urea | 0.9 M, 50% inhibition | Plasmodium falciparum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | activation at 0.4 M, inhibition at higher concentration | Plasmodium falciparum | |
Mg2+ | required | Plasmodium falciparum | |
NaCl | activation at 0.2 M, inhibition at higher concentration | Plasmodium falciparum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
83000 | - |
4 * 83000, SDS-PAGE | Plasmodium falciparum |
330000 | - |
gel filtration | Plasmodium falciparum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Plasmodium falciparum |
Storage Stability | Organism |
---|---|
-20°C or -80°C for 3 months in 20 mM Tris-HCl, pH 8.0 buffer containing 10 or 20% glycerol, no loss of activity. In buffer without glycerol, 30 and 50% of the enzyme activity are lost when stored for 6 months at -80°C and -20°C, respectively | Plasmodium falciparum |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 83000, SDS-PAGE | Plasmodium falciparum |
Synonyms | Comment | Organism |
---|---|---|
hydroxymethyldihydropterin pyrophosphokinase-dihydropteroate synthase | bifunctional enzyme | Plasmodium falciparum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 45 | - |
Plasmodium falciparum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 45 | 30°C: about 40% of maximal activity, 37-45°C: optimum | Plasmodium falciparum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
complete inactivation | Plasmodium falciparum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10 | - |
- |
Plasmodium falciparum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8.5 | 10.5 | pH 8.5: about 40% of maximal activity, pH 10.5: about 90% of maximal activity | Plasmodium falciparum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.011 | - |
sulfadoxine | - |
Plasmodium falciparum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Plasmodium falciparum | calulation from amino acid sequence | - |
7.1 |