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Literature summary for 2.7.6.1 extracted from
- Mammalian phosphoribosyl-pyrophosphate synthetase (1995), Adv. Enzyme Regul., 35, 229-249.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| phosphate | specific and absolute requirement | Homo sapiens |  |
| phosphate | specific and absolute requirement | Rattus norvegicus | |
| sulfate | can partially replace phosphate in activation | Homo sapiens | |
| sulfate | can partially replace phosphate in activation | Rattus norvegicus | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of isoenzymes PRSI and PRSII in Escherichia coli | Homo sapiens |
| expression of isoenzymes PRSI and PRSII in Escherichia coli | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | - |
Homo sapiens | |
| ADP | one of the most effective | Rattus norvegicus | |
| AMP | - |
Homo sapiens | |
| AMP | one of the most effective | Rattus norvegicus | |
| CTP | - |
Homo sapiens | |
| CTP | weak inhibition at 1 mM | Rattus norvegicus | |
| GDP | - |
Homo sapiens | |
| GDP | one of the most effective | Rattus norvegicus | |
| GTP | - |
Homo sapiens | |
| GTP | weak inhibition at 1 mM | Rattus norvegicus | |
| PRPP synthetase-associated proteins | inhibit catalytic and perhaps regulatory functions of the enzyme | Homo sapiens | |
| PRPP synthetase-associated proteins | inhibit catalytic and perhaps regulatory functions of the enzyme | Rattus norvegicus | |
| UDP | 10%-16% inhibition at 1 mM | Homo sapiens | |
| UDP | 10%-16% inhibition at 1 mM | Rattus norvegicus | |
| UMP | 10%-18% inhibition at 1 mM | Homo sapiens | |
| UMP | 10%-18% inhibition at 1 mM | Rattus norvegicus | |
| UTP | weak inhibition at 1 mM | Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.04 | - |
D-ribose 5-phosphate | pH 7.4, 37ºC, isoenzyme rPRSI | Rattus norvegicus | |
| 0.044 | - |
ATP | pH 7.4, 37ºC, isoenzyme rPRSI | Rattus norvegicus | |
| 0.06 | - |
ATP | pH 7.4, 37ºC, isoenzyme rPRSII | Rattus norvegicus | |
| 0.073 | - |
D-ribose 5-phosphate | pH 7.4, 37ºC, isoenzyme rPRSII | Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| brush border | intestinal mucosa | Rattus norvegicus | 5903 | - |
| cytosol | 60% of the total activity, fractionation by discontinuous sucrose density gradient centrifugation | Rattus norvegicus | 5829 | - |
| microsome | 30% of the total activity, fractionation by discontinuous sucrose density gradient centrifugation | Rattus norvegicus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Homo sapiens |
| additional information | - |
the catalytic subunit complexes with the PRPP synthetase-associated protein of 39000 Da, PAP39, to form highly aggregated forms | Rattus norvegicus |
| 34000 | - |
x * 34000 + x * 39000 + x * 41000, SDS-PAGE, the 34000 Da subunit is the catalytic subunit which appears as PRSI or PRSII | Rattus norvegicus |
| 39000 | - |
x * 34000 + x * 39000 + x * 41000, SDS-PAGE, the 34000 Da subunit is the catalytic subunit which appears as PRSI or PRSII | Rattus norvegicus |
| 41000 | - |
x * 34000 + x * 39000 + x * 41000, SDS-PAGE, the 34000 Da subunit is the catalytic subunit which appears as PRSI or PRSII | Rattus norvegicus |
| 68000 | - |
x * 68000, gel filtration in the presence of MgCl2 1 M | Rattus norvegicus |
| 1000000 | - |
- |
Homo sapiens |
| 1000000 | - |
chromatography on DEAE-Toyopearl | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-ribose 5-phosphate | Homo sapiens | the product phosphoribosyldiphosphate is required for the biosynthesis of purine, pyrimidine and pyridine nucleotides, L-histidine and L-tryptophan | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
| ATP + D-ribose 5-phosphate | Rattus norvegicus | the product phosphoribosyldiphosphate is required for the biosynthesis of purine, pyrimidine and pyridine nucleotides, L-histidine and L-tryptophan | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
| Rattus norvegicus | - |
rat | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| adipose tissue | isoenzymes PRSI and PRSII | Rattus norvegicus | - |
| adrenal gland | isoenzyme PRSI | Rattus norvegicus | - |
| brain | isoenzyme PRSI | Rattus norvegicus | - |
| erythrocyte | - |
Homo sapiens | - |
| glioblastoma cell | cell line | Homo sapiens | - |
| liver | - |
Rattus norvegicus | - |
| lung | isoenzyme PRSII | Rattus norvegicus | - |
| spleen | isoenzyme PRSII | Rattus norvegicus | - |
| testis | isoenzymes PRSI and PRSII | Rattus norvegicus | - |
| thymus | isoenzymes PRSI and PRSII | Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Homo sapiens |
| additional information | - |
33400 U/mg and 46200 U/mg for PRSI and PRSII purified isoenzymes, respectively | Rattus norvegicus |
| additional information | - |
purified enzyme has an activity of 7280 U/mg | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-ribose 5-phosphate | - |
Homo sapiens | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
| ATP + D-ribose 5-phosphate | - |
Rattus norvegicus | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
| ATP + D-ribose 5-phosphate | the product phosphoribosyldiphosphate is required for the biosynthesis of purine, pyrimidine and pyridine nucleotides, L-histidine and L-tryptophan | Homo sapiens | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
| ATP + D-ribose 5-phosphate | the product phosphoribosyldiphosphate is required for the biosynthesis of purine, pyrimidine and pyridine nucleotides, L-histidine and L-tryptophan | Rattus norvegicus | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | - |
Homo sapiens |
| ? | x * 34000 + x * 39000 + x * 41000, SDS-PAGE, the 34000 Da subunit is the catalytic subunit which appears as PRSI or PRSII | Rattus norvegicus |
| ? | x * 68000, gel filtration in the presence of MgCl2 1 M | Rattus norvegicus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 49 | - |
- |
Homo sapiens |
| 49 | - |
half-life of 250 min, 90 min and 0.5 min for native enzyme, isoenzymes rPRSI and rPRSII, respectively | Rattus norvegicus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Rattus norvegicus | isoenzyme rPRSII | 4.4 | 4.3 |
| Rattus norvegicus | isoenzyme rPRSI | - |
4.4 |
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