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Literature summary for 2.7.4.9 extracted from

  • Doharey, P.K.; Singh, S.K.; Verma, P.; Verma, A.; Rathaur, S.; Saxena, J.K.
    Insights into the structure-function relationship of Brugia malayi thymidylate kinase (BmTMK) (2016), Int. J. Biol. Macromol., 88, 565-571.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
guanidine hydrochloride guanidine hydrochloride-induced unfolding of thymidylate kinase is noncooperative and influences the functional properties of the enzyme much less than their Cm values. Complete inhibition at 0.4 M Wolbachia endosymbiont of Brugia malayi
Urea urea-induced unfolding of thymidylate kinase is noncooperative and influences the functional properties of the enzyme much less than their Cm values. Complete inhibition at 1.0 M Wolbachia endosymbiont of Brugia malayi

Organism

Organism UniProt Comment Textmining
Wolbachia endosymbiont of Brugia malayi Q5GTD5
-
-
Wolbachia endosymbiont of Brugia malayi TRS Q5GTD5
-
-

Renatured (Commentary)

Renatured (Comment) Organism
guanidine hydrochloride and urea-induced unfolding of thymidylate kinase is noncooperative and influences the functional properties of the enzyme much less than their Cm values. Thymidylate kinase is more prone to ionic perturbation Wolbachia endosymbiont of Brugia malayi

Subunits

Subunits Comment Organism
More the dimeric assembly of thymidylate kinase is an absolute requirement for enzymatic activity and any subtle change in dimeric conformation due to denaturation leads to loss of enzymatic activity Wolbachia endosymbiont of Brugia malayi

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 4 about 100-70% loss of structure Wolbachia endosymbiont of Brugia malayi
5 8 structurally and functionally stable Wolbachia endosymbiont of Brugia malayi
9 10 about 30-40% loss of structure Wolbachia endosymbiont of Brugia malayi

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
155
-
pH 7.4, 37°C Wolbachia endosymbiont of Brugia malayi guanidine hydrochloride
600
-
pH 7.4, 37°C Wolbachia endosymbiont of Brugia malayi Urea