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Literature summary for 2.7.4.8 extracted from

  • Oeschger, M.
    Guanylate kinase from Escherichia coli B (1978), Methods Enzymol., 51, 473-482.
    View publication on PubMed

General Stability

General Stability Organism
unstable in dilute solutions, below 0.005 mg/ml, bovine serum albumin or other suitable proteins protect, highly purified enzyme is stabilized by the presence of lactic dehydrogenase and pyruvate kinase Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3
-
dGMP pH 8.0, 37ºC, NH4+ as activator ion Escherichia coli
0.75
-
Mn2+
-
Escherichia coli
1
-
Mg2+
-
Escherichia coli
1.25
-
Co2+
-
Escherichia coli
10.8 347 K+
-
Escherichia coli
39
-
NH4+
-
Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ activation Escherichia coli
K+ activation Escherichia coli
Mg2+
-
Escherichia coli
Mn2+ requirement Escherichia coli
Mn2+ equally effective as Mg2+ Escherichia coli
Mn2+ 60% as effective as Mg2+ Escherichia coli
NH4+ activation Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
88000
-
equilibrium centrifugation Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli B / ATCC 11303
-
-
-

Purification (Commentary)

Purification (Comment) Organism
method that includes Sephadex and two DEAE-cellulose chromatography Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
187
-
-
Escherichia coli

Storage Stability

Storage Stability Organism
-20°C, stable at all stages of purification Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + dGMP
-
Escherichia coli ADP + dGDP
-
r
ATP + dGMP
-
Escherichia coli B / ATCC 11303 ADP + dGDP
-
r
ATP + GMP specificity Escherichia coli ADP + GDP
-
?
ATP + GMP specificity Escherichia coli B / ATCC 11303 ADP + GDP
-
?
dATP + dGMP
-
Escherichia coli dADP + dGDP
-
?
dATP + dGMP
-
Escherichia coli B / ATCC 11303 dADP + dGDP
-
?
dATP + GMP
-
Escherichia coli dADP + GDP
-
?
dATP + GMP
-
Escherichia coli B / ATCC 11303 dADP + GDP
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3 8.2 Tris-chloride buffer Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
6.5 9 70% of maximal activity at pH 6.5 and 9 Escherichia coli