General Stability | Organism |
---|---|
unstable in dilute solutions, below 0.005 mg/ml, bovine serum albumin or other suitable proteins protect, highly purified enzyme is stabilized by the presence of lactic dehydrogenase and pyruvate kinase | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
dGMP | pH 8.0, 37ºC, NH4+ as activator ion | Escherichia coli | |
0.75 | - |
Mn2+ | - |
Escherichia coli | |
1 | - |
Mg2+ | - |
Escherichia coli | |
1.25 | - |
Co2+ | - |
Escherichia coli | |
10.8 | 347 | K+ | - |
Escherichia coli | |
39 | - |
NH4+ | - |
Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | activation | Escherichia coli | |
K+ | activation | Escherichia coli | |
Mg2+ | - |
Escherichia coli | |
Mn2+ | requirement | Escherichia coli | |
Mn2+ | equally effective as Mg2+ | Escherichia coli | |
Mn2+ | 60% as effective as Mg2+ | Escherichia coli | |
NH4+ | activation | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
88000 | - |
equilibrium centrifugation | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli B / ATCC 11303 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
method that includes Sephadex and two DEAE-cellulose chromatography | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
187 | - |
- |
Escherichia coli |
Storage Stability | Organism |
---|---|
-20°C, stable at all stages of purification | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + dGMP | - |
Escherichia coli | ADP + dGDP | - |
r | |
ATP + dGMP | - |
Escherichia coli B / ATCC 11303 | ADP + dGDP | - |
r | |
ATP + GMP | specificity | Escherichia coli | ADP + GDP | - |
? | |
ATP + GMP | specificity | Escherichia coli B / ATCC 11303 | ADP + GDP | - |
? | |
dATP + dGMP | - |
Escherichia coli | dADP + dGDP | - |
? | |
dATP + dGMP | - |
Escherichia coli B / ATCC 11303 | dADP + dGDP | - |
? | |
dATP + GMP | - |
Escherichia coli | dADP + GDP | - |
? | |
dATP + GMP | - |
Escherichia coli B / ATCC 11303 | dADP + GDP | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.3 | 8.2 | Tris-chloride buffer | Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9 | 70% of maximal activity at pH 6.5 and 9 | Escherichia coli |