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Literature summary for 2.7.4.6 extracted from

  • Tokunaga, H.; Arakawa, T.; Tokunaga, M.
    Cys139Ser mutation in dimeric nucleoside diphosphate kinase generates catalytically competent monomer (2014), Int. J. Biol. Macromol., 66, 66-73.
    View publication on PubMed
Search for more literature for 2.7.4.6

Cloned(Commentary)

Cloned (Comment) Organism
gene ndk, sequence comparison Halomonas sp.
gene ndk, sequence comparison, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21Star (DE3) , subcloning in Escherichia coli strains JM109 and DH5alpha Chromohalobacter salexigens

Protein Variants

Protein Variants Comment Organism
C139S site-directed mutagenesis, the mutation in the dimeric nucleoside diphosphate kinase generates a catalytically competent enzyme monomer Halomonas sp.
C139S site-directed mutagenesis, the mutation in the dimeric nucleoside diphosphate kinase generates a catalytically competent enzyme monomer. Substitution of Cys139 for Ser causes dissociation of dimeric CsNDK into monomer in Tris buffer, and the mutant CsNDK becomes more susceptible to endproteinase GluC cleavage, which is suppressed by an NDK substrate, ATP Chromohalobacter salexigens

Organism

Organism UniProt Comment Textmining
Chromohalobacter salexigens Q1QTL2 gene ndk
-
Chromohalobacter salexigens DSM 3043 Q1QTL2 gene ndk
-
Halomonas sp. Q83WH5
-
-

Subunits

Subunits Comment Organism
dimer wild-type enzyme Chromohalobacter salexigens
dimer wild-type enzyme Halomonas sp.
monomer enzyme mutant C139S Chromohalobacter salexigens
monomer enzyme mutant C139S Halomonas sp.
More three-dimensional structure structure modeling Chromohalobacter salexigens
More three-dimensional structure structure modeling Halomonas sp.

Synonyms

Synonyms Comment Organism
NDK
-
 Chromohalobacter salexigens
NDK
-
 Halomonas sp.
nucleoside diphosphate kinase
-
 Chromohalobacter salexigens
nucleoside diphosphate kinase
-
 Halomonas sp.

General Information

General Information Comment Organism
evolution enzymes from Halomonas sp strain 593 and Chromohalobacter salexigens strain DSM3043 show very high sequence homology to each other, in both enzymes, residue C139 is conserved Chromohalobacter salexigens
evolution enzymes from Halomonas sp strain 593 and Chromohalobacter salexigens strain DSM3043 show very high sequence homology to each other, in both enzymes, residue C139 is conserved Halomonas sp.