Cloned (Comment) | Organism |
---|---|
gene ndk, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha | Acinetobacter baumannii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Urea | complete enzyme inactivation at 6 M, 50% inhibition at 5 M | Acinetobacter baumannii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Acinetobacter baumannii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + nucleoside diphosphate | Acinetobacter baumannii | - |
ADP + nucleoside triphosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter baumannii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis | Acinetobacter baumannii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + nucleoside diphosphate | - |
Acinetobacter baumannii | ADP + nucleoside triphosphate | - |
r | |
ATP + nucleoside diphosphate | analysis of ATP binding by the enzyme at different concentrations and pH values, overview | Acinetobacter baumannii | ADP + nucleoside triphosphate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
NDK | - |
Acinetobacter baumannii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Acinetobacter baumannii |
General Information | Comment | Organism |
---|---|---|
physiological function | nucleoside diphosphate kinase is involved in the catalysis of the transfer of gamma-phosphate from nucleoside triphosphate to nucleoside diphosphate and thus maintains the nucleotide pool. The dNTPs that are formed are used as precursors for DNA and RNA synthesis. NTPs, specifically GTP, are important for cellular macromolecular synthesis and signalling mechanisms. Ndk plays a pivotal role in crucial events like bacterial growth, signal transduction, and pathogenicity | Acinetobacter baumannii |