Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 Star (DE3) cells | Halobacterium salinarum |
Protein Variants | Comment | Organism |
---|---|---|
D148C | the mutation enhances stability and folding in low salt solution by S-S bond. The mutant shows increased thermal stability by about 10°C in 0.2 M NaCl over the wild type enzyme | Halobacterium salinarum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Halobacterium salinarum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
HisTrap column chromatography and Hitrap Q column chromatography | Halobacterium salinarum |
Renatured (Comment) | Organism |
---|---|
refolding of heat-denatured wild type enzyme in 50 mM Tris-HCl (pH 7.5) occurs depending on the salt concentration. Refolding rate is slow even in the presence of 2 M NaCl, reaching maximum at 2 days | Halobacterium salinarum |
Subunits | Comment | Organism |
---|---|---|
hexamer | native enzyme | Halobacterium salinarum |
Synonyms | Comment | Organism |
---|---|---|
NDK | - |
Halobacterium salinarum |
nucleoside diphosphate kinase | - |
Halobacterium salinarum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 80 | after 5 min incubation, the wild type enzyme loses about 30% activity at 30°C and 100% activity at 40°C in 0.2 M NaCl. In 3.8 M NaCl, the wild type enzyme remains stable up to 75°C and then rapidly loses activity at 80°C | Halobacterium salinarum |