Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.4.6 extracted from

  • Tokunaga, H.; Arakawa, T.; Tokunaga, M.
    Engineering of halophilic enzymes: two acidic amino acid residues at the carboxy-terminal region confer halophilic characteristics to Halomonas and Pseudomonas nucleoside diphosphate kinases (2008), Protein Sci., 17, 1603-1610.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.4.6

Protein Variants

Protein Variants Comment Organism
A134E/A135E site-directed mutagenesis, the mutant shows decreased oligomerization compared to the wild-type enzyme Pseudomonas sp.
E134A/E135A site-directed mutagenesis, the mutant shows increased oligomerization compared to the wild-type enzyme Halomonas sp. #593

General Stability

General Stability Organism
NaCl stabilizes the A134E/A135E mutant enzyme, but not the wild-type enzyme Pseudomonas sp.
NaCl stabilizes the wild-type enzyme, but the E134A/E135A mutant Halomonas sp. #593

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Halomonas sp. #593 critical role of the two amino acid residues, E134 and E135, at the carboxy-terminal region of NDK in determining the halophilic characteristics of the protein, overview ?
-
 ?

Organism

Organism UniProt Comment Textmining
Halomonas sp. #593 Q83WH5
-
-
Pseudomonas sp.
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1000
-
 about, in absence of NaCl, purified wild-type enzyme Halomonas sp. #593
1100
-
 about, in absence of NaCl, purified wild-type enzyme Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information critical role of the two amino acid residues, E134 and E135, at the carboxy-terminal region of NDK in determining the halophilic characteristics of the protein, overview Halomonas sp. #593 ?
-
 ?

Subunits

Subunits Comment Organism
dimer the sequence of amino acids A-Y-F-F-E-E-S-E of HaNDK is involved in the tetrameric assembly Halomonas sp. #593
tetramer the sequence of amino acids A-Y-F-F-AA-T-E of PaNDK is involved in the tetrameric assembly Pseudomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Pseudomonas sp.
30
-
 assay at Halomonas sp. #593

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
85
-
 5 min, the enzyme shows irreversible heat denaturation Pseudomonas sp.
85
-
 5 min, the enzyme shows reversible heat denaturation Halomonas sp. #593