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Literature summary for 2.7.4.6 extracted from

  • Johansson, M.; Hammargren, J.; Uppsaell, E.; MacKenzie, A.; Knorpp, C.
    The activities of nucleoside diphosphate kinase and adenylate kinase are influenced by their interaction (2008), Plant Sci., 174, 192-199.
    View publication on PubMed
Search for more literature for 2.7.4.6

Activating Compound

Activating Compound Comment Organism Structure
additional information adenylate kinase from Arabidopsis thaliana stimulates NDPK activity, whereas NDPK inhibits the activity of adenylate kinase, overview Pisum sativum

Cloned(Commentary)

Cloned (Comment) Organism
expression of His-tagged NDPK in Escherichia coli Pisum sativum

Protein Variants

Protein Variants Comment Organism
additional information immobiliztaion of the purified recombinant enzyme on N-hydroxysuccinimide HS-activated HP resin Pisum sativum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.126
-
 ATP recombinant His-tagged NDPK Pisum sativum

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Pisum sativum 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Pisum sativum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + NDP Pisum sativum
-
 ADP + NTP
-
 r
additional information Pisum sativum nucleoside diphosphate kinase is responsible for maintaining the nucleotide balance in the cell, but is also involved in regulatory functions that often are mediated via protein-protein interactions, differentially for adenylate kinase isozymes, overview ?
-
 ?

Organism

Organism UniProt Comment Textmining
Pisum sativum
-
 cv. Oregon sugarpod
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged NDPK from Escherichia coli by nickel affinity chromatography Pisum sativum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + GDP
-
 Pisum sativum ADP + GTP
-
 r
ATP + NDP
-
 Pisum sativum ADP + NTP
-
 r
additional information nucleoside diphosphate kinase is responsible for maintaining the nucleotide balance in the cell, but is also involved in regulatory functions that often are mediated via protein-protein interactions, differentially for adenylate kinase isozymes, overview Pisum sativum ?
-
 ?
additional information protein-protein interaction analysis of adenylate kinases from Arabidopsis thaliana with the immobilized recombinant His-tagged NDPK from Pisum sativum, the interaction influences both enzyme activities, overview Pisum sativum ?
-
 ?

Synonyms

Synonyms Comment Organism
NDPK
-
 Pisum sativum
nucleoside diphosphate kinase
-
 Pisum sativum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2085
-
 ATP recombinant His-tagged NDPK Pisum sativum