Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | nucleoside triphosphate, especially GTP, promotes the dynamic self-assembly of isozyme NDPKB into ordered 20-25 nm diameter filaments in vitro | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression of His-tagged wild-type and mutant NDPKB | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
H118C | the mutant of isozyme NDPKB is catalytically inactive | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + GDP | Homo sapiens | - |
ADP + GTP | - |
? | |
additional information | Homo sapiens | the enzyme catalyzes transfer of the gamma-phosphate from a nucleoside triphosphate, NTP, to a nucleoside diphosphate, NDP | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
isozyme NDPKB | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and mutant NDPKB by nickel affinity chromatography | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + nucleoside diphosphate = ADP + nucleoside triphosphate | isozyme NDPKB shows a ping-pong phospho-transfer mechanism, salt and nucleotide dependent NDPKB assembly is dynamic and requires its catalytic activity | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + GDP | - |
Homo sapiens | ADP + GTP | - |
? | |
additional information | the enzyme catalyzes transfer of the gamma-phosphate from a nucleoside triphosphate, NTP, to a nucleoside diphosphate, NDP | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | wild-type enzyme, but not catalytically inactive H118C mutant isozyme NDPKB, undergoes dynamic self-assembly into ordered 20-25 nm diameter filaments in vitro. Self-assembly is nucleoside triphosphate dependent, GTP being most effective at promoting polymer formation, polymerization also depends on formation of a phosphoryl-His intermediate of the enzyme, mechanism, overview | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
More | the enzyme belongs to the nucleoside diphosphate kinase family | Homo sapiens |
NDPK | - |
Homo sapiens |
nucleoside diphosphate kinase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assembly assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assembly assay at | Homo sapiens |