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Literature summary for 2.7.4.6 extracted from

  • Morin-Leisk, J.; Lee, T.H.
    Nucleotide-dependent self-assembly of nucleoside diphosphate kinase (NDPK) in vitro (2008), Biochim. Biophys. Acta, 1784, 2045-2051.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information nucleoside triphosphate, especially GTP, promotes the dynamic self-assembly of isozyme NDPKB into ordered 20-25 nm diameter filaments in vitro Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression of His-tagged wild-type and mutant NDPKB Homo sapiens

Protein Variants

Protein Variants Comment Organism
H118C the mutant of isozyme NDPKB is catalytically inactive Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + GDP Homo sapiens
-
ADP + GTP
-
?
additional information Homo sapiens the enzyme catalyzes transfer of the gamma-phosphate from a nucleoside triphosphate, NTP, to a nucleoside diphosphate, NDP ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
isozyme NDPKB
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged wild-type and mutant NDPKB by nickel affinity chromatography Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
ATP + nucleoside diphosphate = ADP + nucleoside triphosphate isozyme NDPKB shows a ping-pong phospho-transfer mechanism, salt and nucleotide dependent NDPKB assembly is dynamic and requires its catalytic activity Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + GDP
-
Homo sapiens ADP + GTP
-
?
additional information the enzyme catalyzes transfer of the gamma-phosphate from a nucleoside triphosphate, NTP, to a nucleoside diphosphate, NDP Homo sapiens ?
-
?

Subunits

Subunits Comment Organism
hexamer wild-type enzyme, but not catalytically inactive H118C mutant isozyme NDPKB, undergoes dynamic self-assembly into ordered 20-25 nm diameter filaments in vitro. Self-assembly is nucleoside triphosphate dependent, GTP being most effective at promoting polymer formation, polymerization also depends on formation of a phosphoryl-His intermediate of the enzyme, mechanism, overview Homo sapiens

Synonyms

Synonyms Comment Organism
More the enzyme belongs to the nucleoside diphosphate kinase family Homo sapiens
NDPK
-
Homo sapiens
nucleoside diphosphate kinase
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assembly assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
assembly assay at Homo sapiens