Literature summary for 2.7.4.6 extracted from

Misra, G.; Aggarwal, A.; Mittal, S.; Singh, Y.; Ramachandran, R.
Purification, crystallization and preliminary structural analysis of nucleoside diphosphate kinase from Bacillus anthracis (2007), Acta Crystallogr. Sect. F, 63, 1084-1086.

Search for more literature for 2.7.4.6

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapour-diffusion method, 0.002 ml protein solution consisting of 10 mg/ml protein in 50 mM Tris-HCl, pH 8.0, 50 mM NaCl, 5 mM EDTA equilibrated against 0.5 ml reservoir solution consisting of 2.25 M ammonium formate and 0.1 M HEPES buffer, pH 7.25, X-ray diffraction structure determination at 2.0 A resolution	Bacillus anthracis

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	-	Bacillus anthracis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Bacillus anthracis	the enzyme's primary function is to maintain dNTP pools by converting deoxynucleotide diphosphates to triphosphates using ATP as the major phosphate donor	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Bacillus anthracis	Q81SV8	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	the enzyme's primary function is to maintain dNTP pools by converting deoxynucleotide diphosphates to triphosphates using ATP as the major phosphate donor	Bacillus anthracis	?	-	?

Synonyms

Synonyms	Comment	Organism
NDK	-	Bacillus anthracis
nucleoside diphosphate kinase	-	Bacillus anthracis

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
22	-	assay at room temperature	Bacillus anthracis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.4	-	assay at	Bacillus anthracis

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Bacillus anthracis

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)