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Literature summary for 2.7.4.6 extracted from
- Import and localisation of nucleoside diphosphate kinase 2 in chloroplasts (2007), J. Plant Res., 120, 451-456.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| transient expression of GFP-tagged wild-type and mutant NDPK2s in Pisum sativum protoplasts | Pisum sativum |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| A77T | site-directed mutagenesis, the mutant shows an altered import behaviour into chloroplasts compared to the wild-type enzyme, overview | Pisum sativum |
| R63D | site-directed mutagenesis, the mutant shows an altered import behaviour into chloroplasts compared to the wild-type enzyme, overview | Pisum sativum |
| R74D | site-directed mutagenesis, the mutant shows an altered import behaviour into chloroplasts compared to the wild-type enzyme, overview | Pisum sativum |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | chloroplast stroma | Pisum sativum | 9507 | - |
| chloroplast stroma | NDPK2 contains a predicted chloroplast targeting transit peptide of 63 amino acids, import mechanism, overview | Pisum sativum | 9570 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 17400 | - |
x * 25000, precursor protein, x * 18500, and x * 17400, mature enzyme forms | Pisum sativum |
| 18500 | - |
x * 25000, precursor protein, x * 18500, and x * 17400, mature enzyme forms | Pisum sativum |
| 18500 | - |
x * 18500, mature form, SDS-PAGE, x * 25000, precursor form, SDS-PAGE | Pisum sativum |
| 25000 | - |
x * 25000, precursor protein, x * 18500, and x * 17400, mature enzyme forms | Pisum sativum |
| 25000 | - |
x * 18500, mature form, SDS-PAGE, x * 25000, precursor form, SDS-PAGE | Pisum sativum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Pisum sativum | key enzyme involved in the homeostasis of nucleoside triphosphates | ? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pisum sativum | - |
two mature isoforms of NDPK2 in plastids | - |
| Pisum sativum | P47923 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| proteolytic modification | 25000 Da, precursor protein, 18500 Da and 17400 Da, mature enzyme forms starting at amino acids 65 and 78 resp. | Pisum sativum |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme from chloroplasts to homogeneity, from cytosol and membranes | Pisum sativum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Pisum sativum | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | key enzyme involved in the homeostasis of nucleoside triphosphates | Pisum sativum | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 25000, precursor protein, x * 18500, and x * 17400, mature enzyme forms | Pisum sativum |
| ? | x * 18500, mature form, SDS-PAGE, x * 25000, precursor form, SDS-PAGE | Pisum sativum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NDPK2 | - |
Pisum sativum |
| nucleoside diphosphate kinase 2 | - |
Pisum sativum |
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