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Literature summary for 2.7.4.6 extracted from

  • Yonezawa, Y.; Izutsu, K.; Tokunaga, H.; Maeda, H.; Arakawa, T.; Tokunaga, M.
    Dimeric structure of nucleoside diphosphate kinase from moderately halophilic bacterium: contrast to the tetrameric Pseudomonas counterpart (2007), FEMS Microbiol. Lett., 268, 52-58.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene ndk, functional expression of untagged wild-type enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha Pseudomonas sp.
gene ndk, functional expression of untagged wild-type enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha Halomonas sp.

Inhibitors

Inhibitors Comment Organism Structure
NaCl strong inhibition and destabilization at 2 M Halomonas sp.
NaCl strong inhibition and destabilization at 2 M Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.88
-
GTP pH 8.0 Pseudomonas sp.
1.21
-
GTP pH 8.0, presence of 0.2 M NaCl Pseudomonas sp.
2.13
-
GTP pH 8.0, presence of 1 M NaCl Halomonas sp.
2.17
-
GTP pH 8.0, presence of 0.5 M NaCl Pseudomonas sp.
2.24
-
GTP pH 8.0, presence of 0.2 M NaCl Halomonas sp.
2.62
-
GTP pH 8.0, presence of 0.5 M NaCl Halomonas sp.
2.63
-
GTP pH 8.0 Halomonas sp.
3.26
-
GTP pH 8.0, presence of 1 M NaCl Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
15000
-
4 * 15000, about Pseudomonas sp.
15137
-
2 * 15137 Halomonas sp.
30130
-
light scattering Halomonas sp.
62120
-
light scattering Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Halomonas sp.
-
-
-
Halomonas sp.
-
gene ndk
-
Halomonas sp. 4593
-
-
-
Pseudomonas sp.
-
-
-
Pseudomonas sp.
-
gene ndk
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type enzyme from Escherichia coli strain BL21(DE3) Pseudomonas sp.
recombinant wild-type enzyme from Escherichia coli strain BL21(DE3) Halomonas sp.

Renatured (Commentary)

Renatured (Comment) Organism
the NDK readily refolds and reassembles back to the active dimeric structure, upon heat denaturation at 0.2 M NaCl, as soon as the temperature is lowered Halomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1200
-
about, purified recombinant enzyme Halomonas sp.
1200
-
pH 8.0 Halomonas sp.
1500
-
about, purified recombinant enzyme Pseudomonas sp.
1500
-
pH 8.0 Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + nucleoside diphosphate
-
Pseudomonas sp. GDP + nucleoside triphosphate
-
?
GTP + nucleoside diphosphate
-
Halomonas sp. GDP + nucleoside triphosphate
-
?
GTP + nucleoside diphosphate
-
Halomonas sp. 4593 GDP + nucleoside triphosphate
-
?

Subunits

Subunits Comment Organism
dimer light scattering and chemical cross-linking experiments Halomonas sp.
dimer 2 * 15137 Halomonas sp.
tetramer 4 * 15000, about Pseudomonas sp.

Synonyms

Synonyms Comment Organism
NDK
-
Pseudomonas sp.
NDK
-
Halomonas sp.
nucleoside diphosphate kinase
-
Pseudomonas sp.
nucleoside diphosphate kinase
-
Halomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Halomonas sp.
40 50
-
Halomonas sp.
40 50 sharp decrease in activity above 50°C Pseudomonas sp.
60
-
-
Pseudomonas sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 80 temperature profile, overview Pseudomonas sp.
30 60 temperature profile, overview Halomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
inactivation above Pseudomonas sp.
70
-
reversible inactivation Halomonas sp.
80
-
5 min, 10% residual activity. Majority of protein is irreversibly inactiated Pseudomonas sp.
80
-
60 min, 80% residual activity, 100 min, 60% residual activity Halomonas sp.
90
-
irreversible denaturation at the enzyme's melting temperature Pseudomonas sp.

pI Value

Organism Comment pI Value Maximum pI Value
Pseudomonas sp. calculated
-
4.6
Halomonas sp. calculated
-
4.6