Cloned (Comment) | Organism |
---|---|
gene ndk, functional expression of untagged wild-type enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha | Pseudomonas sp. |
gene ndk, functional expression of untagged wild-type enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha | Halomonas sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NaCl | strong inhibition and destabilization at 2 M | Halomonas sp. | |
NaCl | strong inhibition and destabilization at 2 M | Pseudomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.88 | - |
GTP | pH 8.0 | Pseudomonas sp. | |
1.21 | - |
GTP | pH 8.0, presence of 0.2 M NaCl | Pseudomonas sp. | |
2.13 | - |
GTP | pH 8.0, presence of 1 M NaCl | Halomonas sp. | |
2.17 | - |
GTP | pH 8.0, presence of 0.5 M NaCl | Pseudomonas sp. | |
2.24 | - |
GTP | pH 8.0, presence of 0.2 M NaCl | Halomonas sp. | |
2.62 | - |
GTP | pH 8.0, presence of 0.5 M NaCl | Halomonas sp. | |
2.63 | - |
GTP | pH 8.0 | Halomonas sp. | |
3.26 | - |
GTP | pH 8.0, presence of 1 M NaCl | Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
15000 | - |
4 * 15000, about | Pseudomonas sp. |
15137 | - |
2 * 15137 | Halomonas sp. |
30130 | - |
light scattering | Halomonas sp. |
62120 | - |
light scattering | Pseudomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Halomonas sp. | - |
- |
- |
Halomonas sp. | - |
gene ndk | - |
Halomonas sp. 4593 | - |
- |
- |
Pseudomonas sp. | - |
- |
- |
Pseudomonas sp. | - |
gene ndk | - |
Purification (Comment) | Organism |
---|---|
recombinant wild-type enzyme from Escherichia coli strain BL21(DE3) | Pseudomonas sp. |
recombinant wild-type enzyme from Escherichia coli strain BL21(DE3) | Halomonas sp. |
Renatured (Comment) | Organism |
---|---|
the NDK readily refolds and reassembles back to the active dimeric structure, upon heat denaturation at 0.2 M NaCl, as soon as the temperature is lowered | Halomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1200 | - |
about, purified recombinant enzyme | Halomonas sp. |
1200 | - |
pH 8.0 | Halomonas sp. |
1500 | - |
about, purified recombinant enzyme | Pseudomonas sp. |
1500 | - |
pH 8.0 | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + nucleoside diphosphate | - |
Pseudomonas sp. | GDP + nucleoside triphosphate | - |
? | |
GTP + nucleoside diphosphate | - |
Halomonas sp. | GDP + nucleoside triphosphate | - |
? | |
GTP + nucleoside diphosphate | - |
Halomonas sp. 4593 | GDP + nucleoside triphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | light scattering and chemical cross-linking experiments | Halomonas sp. |
dimer | 2 * 15137 | Halomonas sp. |
tetramer | 4 * 15000, about | Pseudomonas sp. |
Synonyms | Comment | Organism |
---|---|---|
NDK | - |
Pseudomonas sp. |
NDK | - |
Halomonas sp. |
nucleoside diphosphate kinase | - |
Pseudomonas sp. |
nucleoside diphosphate kinase | - |
Halomonas sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Halomonas sp. |
40 | 50 | - |
Halomonas sp. |
40 | 50 | sharp decrease in activity above 50°C | Pseudomonas sp. |
60 | - |
- |
Pseudomonas sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 80 | temperature profile, overview | Pseudomonas sp. |
30 | 60 | temperature profile, overview | Halomonas sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
inactivation above | Pseudomonas sp. |
70 | - |
reversible inactivation | Halomonas sp. |
80 | - |
5 min, 10% residual activity. Majority of protein is irreversibly inactiated | Pseudomonas sp. |
80 | - |
60 min, 80% residual activity, 100 min, 60% residual activity | Halomonas sp. |
90 | - |
irreversible denaturation at the enzyme's melting temperature | Pseudomonas sp. |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Pseudomonas sp. | calculated | - |
4.6 |
Halomonas sp. | calculated | - |
4.6 |