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Literature summary for 2.7.4.6 extracted from
- Amino acid residues involved in autophosphorylation and phosphotransfer activities are distinct in nucleoside diphosphate kinase from Mycobacterium tuberculosis (2004), J. Biol. Chem., 279, 43595-43603.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| wild type and mutant proteins expressed as GST-fusion protein in Escherichia coli BL21(DE3) or DH5alpha, complementation studies with NdK deficient Pseudomonas aeruginosa | Mycobacterium tuberculosis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| H52A | unable to complement NdK deficient Pseudomonas aeruginosa, Km values similar to wild type protein but significantly reduced turnover | Mycobacterium tuberculosis |
| H53Q | unable to complement NdK deficient Pseudomonas aeruginosa, Km values similar to wild type protein but significantly reduced turnover | Mycobacterium tuberculosis |
| K10A | no phosphotransfer activity | Mycobacterium tuberculosis |
| K29A | no phosphotransfer activity | Mycobacterium tuberculosis |
| R104A | no phosphotransfer activity | Mycobacterium tuberculosis |
| R86A | no phosphotransfer activity | Mycobacterium tuberculosis |
| Y50F | unable to complement NdK deficient Pseudomonas aeruginosa, no phosphotransfer activity | Mycobacterium tuberculosis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| AMP | IC50: 1.9 mM | Mycobacterium tuberculosis |  |
| cAMP | IC50: 26.7 mM | Mycobacterium tuberculosis | |
| chromoglycate | IC50: 90 mM | Mycobacterium tuberculosis | |
| UDP | IC50: 1.6 mM | Mycobacterium tuberculosis | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | enzyme is secreted to the culture medium | Mycobacterium tuberculosis | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | divalent cation required for catalysis | Mycobacterium tuberculosis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 16500 | - |
6 * 16500 | Mycobacterium tuberculosis |
| 107000 | - |
gel filtration | Mycobacterium tuberculosis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + dNDP | Mycobacterium tuberculosis | contributes to the maintenance of the cellular pools of all deoxynucleoside triphosphates | ADP + dNTP | - |
? | |
| ATP + dNDP | Mycobacterium tuberculosis H37Ra / ATCC 25177 | contributes to the maintenance of the cellular pools of all deoxynucleoside triphosphates | ADP + dNTP | - |
? | |
| ATP + NDP | Mycobacterium tuberculosis | contributes to the maintenance of the cellular pools of all nucleoside triphosphates | ADP + NTP | - |
? | |
| ATP + NDP | Mycobacterium tuberculosis H37Ra / ATCC 25177 | contributes to the maintenance of the cellular pools of all nucleoside triphosphates | ADP + NTP | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycobacterium tuberculosis | A5U5E1 | - |
- |
| Mycobacterium tuberculosis H37Ra / ATCC 25177 | A5U5E1 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombisnt proteins using GST-tag | Mycobacterium tuberculosis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP + dGTP | - |
Mycobacterium tuberculosis | ATP + dGDP | - |
? | |
| ADP + dGTP | - |
Mycobacterium tuberculosis H37Ra / ATCC 25177 | ATP + dGDP | - |
? | |
| ATP + dNDP | contributes to the maintenance of the cellular pools of all deoxynucleoside triphosphates | Mycobacterium tuberculosis | ADP + dNTP | - |
? | |
| ATP + dNDP | contributes to the maintenance of the cellular pools of all deoxynucleoside triphosphates | Mycobacterium tuberculosis H37Ra / ATCC 25177 | ADP + dNTP | - |
? | |
| ATP + NDP | - |
Mycobacterium tuberculosis | ADP + NTP | - |
? | |
| ATP + NDP | contributes to the maintenance of the cellular pools of all nucleoside triphosphates | Mycobacterium tuberculosis | ADP + NTP | - |
? | |
| ATP + NDP | - |
Mycobacterium tuberculosis H37Ra / ATCC 25177 | ADP + NTP | - |
? | |
| ATP + NDP | contributes to the maintenance of the cellular pools of all nucleoside triphosphates | Mycobacterium tuberculosis H37Ra / ATCC 25177 | ADP + NTP | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | 6 * 16500 | Mycobacterium tuberculosis |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 1.6 | - |
IC50: 1.6 mM | Mycobacterium tuberculosis | UDP | |
| 1.9 | - |
IC50: 1.9 mM | Mycobacterium tuberculosis | AMP | |
| 26.7 | - |
IC50: 26.7 mM | Mycobacterium tuberculosis | cAMP | |
| 90 | - |
IC50: 90 mM | Mycobacterium tuberculosis | chromoglycate | |
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