General Stability | Organism |
---|---|
benzamidine and PMSF stabilize during purification | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.17 | - |
dTDP | cosubstrate ATP, 37°C, pH 7.6 | Saccharomyces cerevisiae | |
0.25 | - |
dGDP | cosubstrate ATP, 37°C, pH 7.6 | Saccharomyces cerevisiae | |
0.26 | 0.37 | CDP | 37°C, pH 7.6 | Saccharomyces cerevisiae | |
0.26 | 0.37 | CDP | cosubstrate ATP | Saccharomyces cerevisiae | |
0.28 | - |
UDP | cosubstrate ATP, 37°C, pH 7.6 | Saccharomyces cerevisiae | |
0.5 | 0.54 | dCDP | cosubstrate ATP, 37°C, pH 7.6 | Saccharomyces cerevisiae | |
0.5 | 0.54 | dUDP | cosubstrate ATP, 37°C, pH 7.6 | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
68000 | 70000 | FPLC gel filtration | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
NDP + NTP | Saccharomyces cerevisiae | involved in RNA- and DNA metabolism | NTP + NDP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
baker's yeast, strain BJ2168 | - |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5.4 | - |
37°C, pH 7.6 | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + ADP | less effective dinucleotide substrate | Saccharomyces cerevisiae | ADP + ATP | - |
? | |
ATP + CDP | highly effective dinucleotide substrate | Saccharomyces cerevisiae | ADP + CTP | - |
? | |
ATP + dADP | less effective dinucleotide substrate | Saccharomyces cerevisiae | ADP + dATP | - |
? | |
ATP + dCDP | less effective dinucleotide substrate | Saccharomyces cerevisiae | ADP + dCTP | - |
? | |
ATP + dGDP | less effective dinucleotide substrate | Saccharomyces cerevisiae | ADP + dGTP | - |
? | |
ATP + dTDP | highly effective dinucleotide substrate | Saccharomyces cerevisiae | ADP + dTTP | - |
? | |
ATP + dUDP | highly effective dinucleotide substrate | Saccharomyces cerevisiae | ? | - |
? | |
ATP + UDP | highly effective dinucleotide substrate | Saccharomyces cerevisiae | ADP + UTP | - |
? | |
GTP + ADP | GDP is less effective dinucleotide substrate | Saccharomyces cerevisiae | GDP + ATP | - |
r | |
NDP + NTP | involved in RNA- and DNA metabolism | Saccharomyces cerevisiae | NTP + NDP | - |
? | |
NTP + nucleoside diphosphate | broad specificity | Saccharomyces cerevisiae | NDP + NTP | - |
? | |
NTP + nucleoside diphosphate | in decreasing order of activity: UTP, dTTP, CTP, dATP, ATP, dGTP, GTP | Saccharomyces cerevisiae | NDP + NTP | - |
? | |
UTP + dTDP | best substrates | Saccharomyces cerevisiae | UDP + dTDP | - |
? | |
UTP + dTDP | nucleoside triphosphates in decreasing order of activity: dTTP, CTP, dCTP, dATP, dGTP, GTP | Saccharomyces cerevisiae | UDP + dTDP | - |
? | |
UTP + dTDP | nucleoside diphosphates in decreasing order of activity: CDP, UDP, GDP, dGDP, dCDP, dADP, ADP | Saccharomyces cerevisiae | UDP + dTDP | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 17000-18000, SDS-PAGE | Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
13.3 | - |
dTDP | - |
Saccharomyces cerevisiae |