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Literature summary for 2.7.4.6 extracted from
- Metabolism of deoxyribonucleotides. 3. Purification and some properties of nucleoside diphosphokinase of calf thymus (1966), J. Biol. Chem., 241, 4917-4922.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.21 | 0.39 | dTDP | - |
Bos taurus |  |
| 0.5 | 0.54 | CDP | pH 7.5, 37°C | Bos taurus | |
| 0.5 | 0.54 | CDP | cosubstrate ATP | Bos taurus | |
| 0.56 | - |
dCDP | cosubstrate ATP, pH 7.5, 37°C | Bos taurus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | in decreasing order of activity: Mn2+, Mg2+, Co2+, Zn2+, Ni2+, Ca2+, Fe2+ | Bos taurus | |
| Co2+ | requirement | Bos taurus | |
| Co2+ | in decreasing order of activity: Mn2+, Mg2+, Co2+, Zn2+, Ni2+, Ca2+, Fe2+ | Bos taurus | |
| Fe2+ | requirement, in decreasing order of activity: Mn2+, Mg2+, Co2+, Zn2+, Ni2+, Ca2+, Fe2+ | Bos taurus | |
| Mg2+ | requirement | Bos taurus | |
| Mg2+ | in decreasing order of activity: Mn2+, Mg2+, Co2+, Zn2+, Ni2+, Ca2+, Fe2+ | Bos taurus | |
| Mn2+ | requirement | Bos taurus | |
| Mn2+ | in decreasing order of activity: Mn2+, Mg2+, Co2+, Zn2+, Ni2+, Ca2+, Fe2+ | Bos taurus | |
| additional information | no activation by Fe3+ | Bos taurus | |
| Ni2+ | requirement, in decreasing order of activity: Mn2+, Mg2+, Co2+, Zn2+, Ni2+, Ca2+, Fe2+ | Bos taurus | |
| Zn2+ | requirement, in decreasing order of activity: Mn2+, Mg2+, Co2+, Zn2+, Ni2+, Ca2+, Fe2+ | Bos taurus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
calf | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| thymus | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Bos taurus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 2 months | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + dTDP | in decreasing order of activity: dGDP, IDP, UDP, dUDP, CDP, GDP, dCDP | Bos taurus | ADP + dTTP | - |
? | |
| ATP + dTDP | best substrate: dTDP | Bos taurus | ADP + dTTP | - |
? | |
| GTP + ADP | dADP can replace ADP | Bos taurus | GDP + ATP | - |
r | |
| NTP + nucleoside diphosphate | broad specificity | Bos taurus | NDP + NTP | - |
? | |
| NTP + nucleoside diphosphate | no substrates are several nucleoside monophosphates | Bos taurus | NDP + NTP | - |
? | |
| UTP + CDP | best substrate: UTP | Bos taurus | UDP + CTP | - |
? | |
| UTP + CDP | in decreasing order of activity: dATP, ITP, dTTP, ATP, dGTP, GTP, dCTP, CTP | Bos taurus | UDP + CTP | - |
? | |
| UTP + dCDP | best substrate: UTP | Bos taurus | UDP + dCTP | - |
? | |
| UTP + dCDP | in decreasing order of activity: ITP, dATP, dTTP, dGTP, GTP, ATP, CTP, dCTP | Bos taurus | UDP + dCTP | - |
? | |
| UTP + dTDP | best substrate: UTP | Bos taurus | UDP + dTDP | - |
? | |
| UTP + dTDP | in decreasing order of activity: ITP, dATP, dTTP, GTP, ATP, dGTP, dUTP, CTP, dCTP | Bos taurus | UDP + dTDP | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Bos taurus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 10.3 | about half-maximal activity at pH 6.5 and 10.3 | Bos taurus |
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