Literature summary for 2.7.4.3 extracted from

Drakou, C.E.; Malekkou, A.; Hayes, J.M.; Lederer, C.W.; Leonidas, D.D.; Oikonomakos, N.G.; Lamond, A.I.; Santama, N.; Zographos, S.E.
hCINAP is an atypical mammalian nuclear adenylate kinase with an ATPase motif: structural and functional studies (2012), Proteins, 80, 206-220.

Search for more literature for 2.7.4.3

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli B834(DE3)pLysS cells	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
in complex with ADP, dADP, and Mg2+ADP-PO43-, hanging drop vapor diffusion method, using 0.1 M HEPES pH 7.5, 1.5 M Li2SO4, 0.2 M NaCl, 0.5 mM dithiothreitol, and 25 mM MgCl2	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
H79G	the mutation affects both adenylate kinase (enzymatic efficiency (kcat/Km) is reduced by 72% relative to the wild type enzyme) and ATPase catalytic efficiency and induces homodimer formation	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
P1,P5-diadenosine 5'-pentaphosphate	adenylate kinase-specific inhibitor, potent inhibitor of CINAP	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.045	-	ATP	wild type enzyme CINAP, in 100 mM Tris-HCl, pH 7.5, 60 mM KCl, temperature not specified in the publication	Homo sapiens
0.093	-	ATP	mutant enzyme CINAP H79G, in 100 mM Tris-HCl, pH 7.5, 60 mM KCl, temperature not specified in the publication	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q9Y3D8	-	-

Purification (Commentary)

Purification (Comment)	Organism
GSTrap 4B column chromatography and Superdex 75 gel filtration	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
HeLa cell	-	Homo sapiens	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.011	-	mutant enzyme CINAP H79G, in 100 mM Tris-HCl, pH 7.5, 60 mM KCl, temperature not specified in the publication	Homo sapiens
0.018	-	wild type enzyme CINAP, in 100 mM Tris-HCl, pH 7.5, 60 mM KCl, temperature not specified in the publication	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + AMP	-	Homo sapiens	ADP + ADP	-	r
additional information	CINAP has previously been designated as an adenylate kinase AK6, but is very atypical as it exhibits unusually broad substrate specificity, structural features characteristic of ATPase/GTPase proteins (Walker motifs A and B) and also intrinsic ATPase activity	Homo sapiens	?	-	?

Synonyms

Synonyms	Comment	Organism
AK6	-	Homo sapiens
CINAP	-	Homo sapiens
coilin interacting nuclear ATPase protein	-	Homo sapiens
phosphotransferase	-	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.0037	-	ATP	mutant enzyme CINAP H79G, in 100 mM Tris-HCl, pH 7.5, 60 mM KCl, temperature not specified in the publication	Homo sapiens
0.0063	-	ATP	wild type enzyme CINAP, in 100 mM Tris-HCl, pH 7.5, 60 mM KCl, temperature not specified in the publication	Homo sapiens

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.00002703	-	P1,P5-diadenosine 5'-pentaphosphate	wild type enzyme CINAP, in 100 mM Tris-HCl, pH 7.5, 60 mM KCl, temperature not specified in the publication	Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
39000	-	ATP	mutant enzyme CINAP H79G, in 100 mM Tris-HCl, pH 7.5, 60 mM KCl, temperature not specified in the publication	Homo sapiens
140000	-	ATP	wild type enzyme CINAP, in 100 mM Tris-HCl, pH 7.5, 60 mM KCl, temperature not specified in the publication	Homo sapiens

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Release 2023.1 (January 2023)