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Literature summary for 2.7.4.3 extracted from

  • Kladova, A.V.; Gavel, O.Y.; Zhadan, G.G.; Roig, M.G.; Shnyrov, V.L.; Bursakov, S.A.
    Zinc-, cobalt- and iron-chelated forms of adenylate kinase from the Gram-negative bacterium Desulfovibrio gigas (2009), Int. J. Biol. Macromol., 45, 524-531.
    View publication on PubMed
Search for more literature for 2.7.4.3

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Megalodesulfovibrio gigas

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.034
-
 ATP Zn2+-containing recombinant adenylate kinase, in 50 mM Tris-HCl, pH 7.6, 100 mM KCl, 0.25 mM MgCl2 Megalodesulfovibrio gigas
0.04
-
 AMP Co2+-containing recombinant adenylate kinase, in 50 mM Tris-HCl, pH 7.6, 100 mM KCl, 0.25 mM MgCl2 Megalodesulfovibrio gigas
0.046
-
 AMP Zn2+-containing recombinant adenylate kinase, in 50 mM Tris-HCl, pH 7.6, 100 mM KCl, 0.25 mM MgCl2 Megalodesulfovibrio gigas
0.049
-
 ATP Co2+-containing recombinant adenylate kinase, in 50 mM Tris-HCl, pH 7.6, 100 mM KCl, 0.25 mM MgCl2 Megalodesulfovibrio gigas
0.071
-
 AMP Fe2+-containing recombinant adenylate kinase, in 50 mM Tris-HCl, pH 7.6, 100 mM KCl, 0.25 mM MgCl2 Megalodesulfovibrio gigas
0.076
-
 ATP Fe2+-containing recombinant adenylate kinase, in 50 mM Tris-HCl, pH 7.6, 100 mM KCl, 0.25 mM MgCl2 Megalodesulfovibrio gigas
0.128
-
 ADP Fe2+-containing recombinant adenylate kinase, in 50 mM Tris-HCl, pH 7.6, 100 mM KCl, 0.25 mM MgCl2 Megalodesulfovibrio gigas
0.165
-
 ADP Zn2+-containing recombinant adenylate kinase, in 50 mM Tris-HCl, pH 7.6, 100 mM KCl, 0.25 mM MgCl2 Megalodesulfovibrio gigas
0.247
-
 ADP Co2+-containing recombinant adenylate kinase, in 50 mM Tris-HCl, pH 7.6, 100 mM KCl, 0.25 mM MgCl2 Megalodesulfovibrio gigas

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ the recombinant enzyme can contain Co2+ Megalodesulfovibrio gigas
Fe2+ the recombinant enzyme can contain Fe2+ Megalodesulfovibrio gigas
Mg2+ required for activity Megalodesulfovibrio gigas
Zn2+ the recombinant enzyme can contain Zn2+ Megalodesulfovibrio gigas

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24700
-
 estimated from SDS-PAGE Megalodesulfovibrio gigas

Organism

Organism UniProt Comment Textmining
Megalodesulfovibrio gigas C7U112
-
-

Purification (Commentary)

Purification (Comment) Organism
Blue Sepharose column chromatography and Superdex 75 gel filtration Megalodesulfovibrio gigas

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + AMP
-
 Megalodesulfovibrio gigas ADP + ADP
-
 ?

Synonyms

Synonyms Comment Organism
ATP:AMP phosphotransferase
-
 Megalodesulfovibrio gigas

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
32
-
 temperature optimum of Fe2+-containing adenylate kinase Megalodesulfovibrio gigas
36
-
 temperature optima of Zn2+- and Co2+-containing adenylate kinase Megalodesulfovibrio gigas

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
43.7 45.3 the melting temperatures of Zn2+-, Co2+-, and Fe2+-containing recombinant adenylate kinases are at 45.3, 43.7, and 45.0°C, respectively Megalodesulfovibrio gigas