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Literature summary for 2.7.4.3 extracted from

  • Storey, K.B.
    Purification and properties of squid mantle adenylate kinase. Role of NADH in control of the enzyme (1976), J. Biol. Chem., 251, 7810-7815.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
(NH4)2SO4 inhibitory above 30 mM, activates below Doryteuthis pealeii
2-mercaptoethanol activation Doryteuthis pealeii
citrate not Doryteuthis pealeii
dithiothreitol activation Doryteuthis pealeii
KCl activation Doryteuthis pealeii
L-cysteine activation Doryteuthis pealeii
NH4Cl activation Doryteuthis pealeii

General Stability

General Stability Organism
NADH or MgATP2- or ATP plus AMP protect against proteolysis by pronase or trypsin and against heat denaturation Doryteuthis pealeii
unstable in dilute solutions and in the absence of SH-compounds Doryteuthis pealeii

Inhibitors

Inhibitors Comment Organism Structure
(NH4)2SO4 above 30 mM, activates below Doryteuthis pealeii
arginine phosphate weak Doryteuthis pealeii
LiCl
-
Doryteuthis pealeii
additional information not inhibitory: citrate Doryteuthis pealeii
Na2SO4
-
Doryteuthis pealeii
NAD+
-
Doryteuthis pealeii
NADH
-
Doryteuthis pealeii
NH4Cl
-
Doryteuthis pealeii
phosphoenolpyruvate
-
Doryteuthis pealeii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.13
-
ATP
-
Doryteuthis pealeii
0.15
-
2 ADP cosubstrate AMP Doryteuthis pealeii
0.15
-
2 ADP ADP in form of MgADP- Doryteuthis pealeii
0.65 0.7 ADP
-
Doryteuthis pealeii

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Doryteuthis pealeii 5829
-
mitochondrion
-
Doryteuthis pealeii 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Doryteuthis pealeii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
gel filtration Doryteuthis pealeii

Organism

Organism UniProt Comment Textmining
Doryteuthis pealeii
-
squid
-

Purification (Commentary)

Purification (Comment) Organism
no isozymes Doryteuthis pealeii

Source Tissue

Source Tissue Comment Organism Textmining
muscle mantle muscle Doryteuthis pealeii
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2700
-
-
Doryteuthis pealeii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 ADP
-
Doryteuthis pealeii ATP + AMP
-
?
ADP + ADP
-
Doryteuthis pealeii ATP + AMP
-
r
ATP + AMP
-
Doryteuthis pealeii ADP + ADP
-
r

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
39
-
t1/2: 11 min Doryteuthis pealeii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
AMP + ATP Doryteuthis pealeii
8.2
-
ADP + ADP Doryteuthis pealeii

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5
-
unstable at Doryteuthis pealeii