Activating Compound | Comment | Organism | Structure |
---|---|---|---|
(NH4)2SO4 | inhibitory above 30 mM, activates below | Doryteuthis pealeii | |
2-mercaptoethanol | activation | Doryteuthis pealeii | |
citrate | not | Doryteuthis pealeii | |
dithiothreitol | activation | Doryteuthis pealeii | |
KCl | activation | Doryteuthis pealeii | |
L-cysteine | activation | Doryteuthis pealeii | |
NH4Cl | activation | Doryteuthis pealeii |
General Stability | Organism |
---|---|
NADH or MgATP2- or ATP plus AMP protect against proteolysis by pronase or trypsin and against heat denaturation | Doryteuthis pealeii |
unstable in dilute solutions and in the absence of SH-compounds | Doryteuthis pealeii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(NH4)2SO4 | above 30 mM, activates below | Doryteuthis pealeii | |
arginine phosphate | weak | Doryteuthis pealeii | |
LiCl | - |
Doryteuthis pealeii | |
additional information | not inhibitory: citrate | Doryteuthis pealeii | |
Na2SO4 | - |
Doryteuthis pealeii | |
NAD+ | - |
Doryteuthis pealeii | |
NADH | - |
Doryteuthis pealeii | |
NH4Cl | - |
Doryteuthis pealeii | |
phosphoenolpyruvate | - |
Doryteuthis pealeii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.13 | - |
ATP | - |
Doryteuthis pealeii | |
0.15 | - |
2 ADP | cosubstrate AMP | Doryteuthis pealeii | |
0.15 | - |
2 ADP | ADP in form of MgADP- | Doryteuthis pealeii | |
0.65 | 0.7 | ADP | - |
Doryteuthis pealeii |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Doryteuthis pealeii | 5829 | - |
mitochondrion | - |
Doryteuthis pealeii | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Doryteuthis pealeii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27000 | - |
gel filtration | Doryteuthis pealeii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Doryteuthis pealeii | - |
squid | - |
Purification (Comment) | Organism |
---|---|
no isozymes | Doryteuthis pealeii |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
muscle | mantle muscle | Doryteuthis pealeii | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2700 | - |
- |
Doryteuthis pealeii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ADP | - |
Doryteuthis pealeii | ATP + AMP | - |
? | |
ADP + ADP | - |
Doryteuthis pealeii | ATP + AMP | - |
r | |
ATP + AMP | - |
Doryteuthis pealeii | ADP + ADP | - |
r |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
39 | - |
t1/2: 11 min | Doryteuthis pealeii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
AMP + ATP | Doryteuthis pealeii |
8.2 | - |
ADP + ADP | Doryteuthis pealeii |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
unstable at | Doryteuthis pealeii |