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Literature summary for 2.7.4.3 extracted from
- Adenylate kinases from thermosensitive Escherichia coli strains (1989), J. Mol. Biol., 207, 151-162.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| wild-type and thermosensitive mutants | Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| P87S | thermosensitive enzyme, about 50% of wild type activity | Escherichia coli |
| S129F | about 25% of wild type activity | Escherichia coli |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | requirement | Escherichia coli |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 129 | - |
mutant S129F | Escherichia coli |
| 250 | - |
mutant P87S | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP + ADP | - |
Escherichia coli | ATP + AMP | - |
r | |
| ATP + AMP | - |
Escherichia coli | ADP + ADP | - |
r | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
thermostability of mutant enzymes | Escherichia coli |
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Release 2023.1 (January 2023)
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