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Literature summary for 2.7.4.26 extracted from

  • Chen, M.; Poulter, C.D.
    Characterization of thermophilic archaeal isopentenyl phosphate kinases. (2010), Biochemistry, 49, 207-217.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.4.26

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0027
-
 isopentenyl diphosphate pH 7.5, 37°C Thermoplasma acidophilum
0.0031
-
 ADP pH 7.5, 37°C Thermoplasma acidophilum
0.0044
-
 isopentenyl phosphate pH 7.5, 37°C Thermoplasma acidophilum
0.006
-
 ATP pH 7.5, 37°C Thermoplasma acidophilum
0.0076
-
 isopentenyl diphosphate pH 7.5, 37°C Methanothermobacter thermautotrophicus
0.0127
-
 isopentenyl phosphate pH 7.5, 37°C Methanothermobacter thermautotrophicus
0.013
-
 ADP pH 7.5, 37°C Methanothermobacter thermautotrophicus
0.0134
-
 ATP pH 7.5, 37°C Methanothermobacter thermautotrophicus
0.0145
-
 isopentenyl thiolophosphate pH 7.5, 37°C Thermoplasma acidophilum
0.0237
-
 isopentenyl thiolophosphate pH 7.5, 37°C Methanothermobacter thermautotrophicus
0.0263
-
 butyl phosphate pH 7.5, 37°C Methanothermobacter thermautotrophicus
0.0297
-
 but-3-en-1-yl phosphate pH 7.5, 37°C Methanothermobacter thermautotrophicus
0.0432
-
 dimethylallyl phosphate pH 7.5, 37°C Methanothermobacter thermautotrophicus
0.103
-
 but-3-en-1-yl phosphate pH 7.5, 37°C Thermoplasma acidophilum
0.173
-
 butyl phosphate pH 7.5, 37°C Thermoplasma acidophilum
0.174
-
 dimethylallyl phosphate pH 7.5, 37°C Thermoplasma acidophilum
0.74
-
 geranyl phosphate pH 7.5, 37°C Methanothermobacter thermautotrophicus
4.7
-
 geranyl phosphate pH 7.5, 37°C Thermoplasma acidophilum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + isopentenyl phosphate Thermoplasma acidophilum
-
 ADP + isopentenyl diphosphate
-
 ?
ATP + isopentenyl phosphate Methanothermobacter thermautotrophicus
-
 ADP + isopentenyl diphosphate
-
 ?
ATP + isopentenyl phosphate Methanothermobacter thermautotrophicus Delta H
-
 ADP + isopentenyl diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Methanothermobacter thermautotrophicus O26153
-
-
Methanothermobacter thermautotrophicus Delta H O26153
-
-
Thermoplasma acidophilum Q9HLX1
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + but-3-en-1-yl phosphate low reaction rate Thermoplasma acidophilum ADP + but-3-en-1-yl diphosphate
-
 ?
ATP + but-3-en-1-yl phosphate low reaction rate Methanothermobacter thermautotrophicus ADP + but-3-en-1-yl diphosphate
-
 ?
ATP + but-3-en-1-yl phosphate low reaction rate Methanothermobacter thermautotrophicus Delta H ADP + but-3-en-1-yl diphosphate
-
 ?
ATP + butyl phosphate low reaction rate Thermoplasma acidophilum ADP + butyl diphosphate
-
 ?
ATP + butyl phosphate low reaction rate Methanothermobacter thermautotrophicus ADP + butyl diphosphate
-
 ?
ATP + butyl phosphate low reaction rate Methanothermobacter thermautotrophicus Delta H ADP + butyl diphosphate
-
 ?
ATP + dimethylallyl phosphate low reaction rate Thermoplasma acidophilum ADP + dimethylallyl diphosphate
-
 ?
ATP + dimethylallyl phosphate low reaction rate Methanothermobacter thermautotrophicus ADP + dimethylallyl diphosphate
-
 ?
ATP + dimethylallyl phosphate low reaction rate Methanothermobacter thermautotrophicus Delta H ADP + dimethylallyl diphosphate
-
 ?
ATP + geranyl phosphate low reaction rate Thermoplasma acidophilum ADP + geranyl diphosphate
-
 ?
ATP + geranyl phosphate low reaction rate Methanothermobacter thermautotrophicus ADP + geranyl diphosphate
-
 ?
ATP + isopentenyl phosphate
-
 Thermoplasma acidophilum ADP + isopentenyl diphosphate
-
 ?
ATP + isopentenyl phosphate
-
 Thermoplasma acidophilum ADP + isopentenyl diphosphate
-
 r
ATP + isopentenyl phosphate
-
 Methanothermobacter thermautotrophicus ADP + isopentenyl diphosphate
-
 ?
ATP + isopentenyl phosphate
-
 Methanothermobacter thermautotrophicus ADP + isopentenyl diphosphate
-
 r
ATP + isopentenyl phosphate
-
 Methanothermobacter thermautotrophicus Delta H ADP + isopentenyl diphosphate
-
 ?
ATP + isopentenyl phosphate
-
 Methanothermobacter thermautotrophicus Delta H ADP + isopentenyl diphosphate
-
 r
ATP + isopentenyl thiolophosphate low reaction rate Thermoplasma acidophilum ADP + isopentenyl thiolodiphosphate
-
 ?
ATP + isopentenyl thiolophosphate low reaction rate Methanothermobacter thermautotrophicus ADP + isopentenyl thiolodiphosphate
-
 ?

Subunits

Subunits Comment Organism
homodimer
-
 Thermoplasma acidophilum
homodimer
-
 Methanothermobacter thermautotrophicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
 Thermoplasma acidophilum
70
-
-
 Methanothermobacter thermautotrophicus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
55 75
-
 Thermoplasma acidophilum
55 75
-
 Methanothermobacter thermautotrophicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 stable up to Methanothermobacter thermautotrophicus
70
-
 stable up to Thermoplasma acidophilum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0142
-
 geranyl phosphate pH 7.5, 37°C Methanothermobacter thermautotrophicus
0.047
-
 geranyl phosphate pH 7.5, 37°C Thermoplasma acidophilum
2.75
-
 isopentenyl diphosphate pH 7.5, 37°C, reverse reaction Thermoplasma acidophilum
2.84
-
 isopentenyl thiolophosphate pH 7.5, 37°C Thermoplasma acidophilum
4.5
-
 isopentenyl diphosphate pH 7.5, 37°C, reverse reaction Methanothermobacter thermautotrophicus
8
-
 isopentenyl phosphate pH 7.5, 37°C Thermoplasma acidophilum
8.07
-
 but-3-en-1-yl phosphate pH 7.5, 37°C Thermoplasma acidophilum
8.22
-
 isopentenyl thiolophosphate pH 7.5, 37°C Methanothermobacter thermautotrophicus
9.9
-
 butyl phosphate pH 7.5, 37°C Thermoplasma acidophilum
11.3
-
 dimethylallyl phosphate pH 7.5, 37°C Thermoplasma acidophilum
20.5
-
 but-3-en-1-yl phosphate pH 7.5, 37°C Methanothermobacter thermautotrophicus
27.5
-
 isopentenyl phosphate pH 7.5, 37°C Methanothermobacter thermautotrophicus
31.8
-
 butyl phosphate pH 7.5, 37°C Methanothermobacter thermautotrophicus
34
-
 dimethylallyl phosphate pH 7.5, 37°C Methanothermobacter thermautotrophicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.7
-
-
 Thermoplasma acidophilum
8.5
-
 broad optimum Methanothermobacter thermautotrophicus

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8.4 less than 50% of maximal activity above and below Thermoplasma acidophilum
7 9 less than 50% of maximal activity above and below Methanothermobacter thermautotrophicus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.01
-
 geranyl phosphate pH 7.5, 37°C Thermoplasma acidophilum
0.19
-
 geranyl phosphate pH 7.5, 37°C Methanothermobacter thermautotrophicus
5.7
-
 butyl phosphate pH 7.5, 37°C Thermoplasma acidophilum
6.5
-
 dimethylallyl phosphate pH 7.5, 37°C Thermoplasma acidophilum
7.8
-
 but-3-en-1-yl phosphate pH 7.5, 37°C Thermoplasma acidophilum
69
-
 but-3-en-1-yl phosphate pH 7.5, 37°C Methanothermobacter thermautotrophicus
120
-
 butyl phosphate pH 7.5, 37°C Methanothermobacter thermautotrophicus
200
-
 isopentenyl thiolophosphate pH 7.5, 37°C Thermoplasma acidophilum
340
-
 ADP pH 7.5, 37°C Methanothermobacter thermautotrophicus
350
-
 isopentenyl thiolophosphate pH 7.5, 37°C Methanothermobacter thermautotrophicus
590
-
 isopentenyl phosphate pH 7.5, 37°C Methanothermobacter thermautotrophicus
790
-
 dimethylallyl phosphate pH 7.5, 37°C Methanothermobacter thermautotrophicus
1000
-
 isopentenyl phosphate pH 7.5, 37°C Thermoplasma acidophilum
1200
-
 ADP pH 7.5, 37°C Thermoplasma acidophilum
1300
-
 ATP pH 7.5, 37°C Thermoplasma acidophilum
1800
-
 isopentenyl phosphate pH 7.5, 37°C Thermoplasma acidophilum
2200
-
 ATP pH 7.5, 37°C Methanothermobacter thermautotrophicus
2200
-
 isopentenyl phosphate pH 7.5, 37°C Methanothermobacter thermautotrophicus