Application | Comment | Organism |
---|---|---|
drug development | the enzyme is found only in bacteria and is a target for the discovery of antibacterials | Staphylococcus aureus |
Cloned (Comment) | Organism |
---|---|
gene pyrH, recombinant expression in Escherichia coli strain BL21 (DE3) from vector pT73.3 | Staphylococcus aureus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-[2-[(2-amino-6-phenylpyrimidin-4-yl)amino]phenyl]ethanone | - |
Staphylococcus aureus | |
2-amino-8-(2-oxo-2-phenylethoxy)-1,4,5,9-tetrahydro-6H-purin-6-one | 10 micromol, 60 min, room temperature, 75% inhibition, crystal enzyme complex structure, overview | Staphylococcus aureus | |
2-amino-8-hydroxy-1,4,5,9-tetrahydro-6H-purin-6-one | 10 micromol, 60 min, room temperature, 18% inhibition | Staphylococcus aureus | |
2-amino-8-[2-(2-methylphenyl)-2-oxoethoxy]-1,4,5,9-tetrahydro-6H-purin-6-one | 10 micromol, 60 min, room temperature, 48% inhibition | Staphylococcus aureus | |
2-amino-8-[2-(3-methylphenyl)-2-oxoethoxy]-1,4,5,9-tetrahydro-6H-purin-6-one | 10 micromol, 60 min, room temperature, 77% inhibition | Staphylococcus aureus | |
2-amino-8-[2-(4-fluorophenyl)-2-oxoethoxy]-1,4,5,9-tetrahydro-6H-purin-6-one | 10 micromol, 60 min, room temperature, 73% inhibition | Staphylococcus aureus | |
2-amino-8-[2-(4-hydroxyphenyl)-2-oxoethoxy]-1,4,5,9-tetrahydro-6H-purin-6-one | 10 micromol, 60 min, room temperature, 77% inhibition | Staphylococcus aureus | |
2-amino-8-[2-(4-methoxyphenyl)-2-oxoethoxy]-1,4,5,9-tetrahydro-6H-purin-6-one | 10 micromol, 60 min, room temperature, 74% inhibition | Staphylococcus aureus | |
2-amino-8-[2-(4-methylphenyl)-2-oxoethoxy]-1,4,5,9-tetrahydro-6H-purin-6-one | 10 micromol, 60 min, room temperature, 80% inhibition | Staphylococcus aureus | |
4-[(2-amino-6-oxo-4,5,6,9-tetrahydro-1H-purin-8-yl)methyl]benzonitrile | 10 micromol, 60 min, room temperature, 53% inhibition, crystal enzyme complex structure, overview | Staphylococcus aureus | |
4-[[(2-amino-6-oxo-4,5,6,9-tetrahydro-1H-purin-8-yl)oxy]acetyl]benzonitrile | 10 micromol, 60 min, room temperature, 68% inhibition | Staphylococcus aureus | |
N4,6-diphenylpyrimidine-2,4-diamine | - |
Staphylococcus aureus | |
N4-(2-methoxyphenyl)-6-phenylpyrimidine-2,4-diamine | - |
Staphylococcus aureus | |
N4-(2-methylphenyl)-6-phenylpyrimidine-2,4-diamine | - |
Staphylococcus aureus | |
N4-(3-methylphenyl)-6-phenylpyrimidine-2,4-diamine | - |
Staphylococcus aureus | |
N4-(4-methoxyphenyl)-6-phenylpyrimidine-2,4-diamine | - |
Staphylococcus aureus | |
N4-(4-methylphenyl)-6-phenylpyrimidine-2,4-diamine | - |
Staphylococcus aureus | |
N4-ethyl-N4,6-diphenylpyrimidine-2,4-diamine | - |
Staphylococcus aureus | |
N6-[(4-nitrophenyl)methyl]-N2-[[3-(trifluoromethyl)phenyl]methyl]-1H-purine-2,6-diamine | - |
Staphylococcus aureus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetics, velocity curves for the enzyme fit to a steady state random Bi Bi mechanism or ordered Bi Bi mechanism. Data fits both models equally well | Staphylococcus aureus | |
0.043 | - |
UMP | pH 7.5, 22°C, random BiBi mechanism | Staphylococcus aureus | |
0.086 | - |
UMP | pH 7.5, 22°C, ordered BiBi mechanism | Staphylococcus aureus | |
0.742 | - |
ATP | pH 7.5, 22°C, random BiBi mechanism | Staphylococcus aureus | |
1.485 | - |
ATP | pH 7.5, 22°C, ordered BiBi mechanism | Staphylococcus aureus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Staphylococcus aureus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + UMP | Staphylococcus aureus | - |
ADP + UDP | - |
r | |
ATP + UMP | Staphylococcus aureus ATCC 601055 | - |
ADP + UDP | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | - |
gene pyrH | - |
Staphylococcus aureus ATCC 601055 | - |
gene pyrH | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + UMP | - |
Staphylococcus aureus | ADP + UDP | - |
r | |
ATP + UMP | UMP kinase catalyzes the transfer of the gamma-phosphate of ATP to UMP | Staphylococcus aureus | ADP + UDP | - |
r | |
ATP + UMP | - |
Staphylococcus aureus ATCC 601055 | ADP + UDP | - |
r | |
ATP + UMP | UMP kinase catalyzes the transfer of the gamma-phosphate of ATP to UMP | Staphylococcus aureus ATCC 601055 | ADP + UDP | - |
r | |
additional information | enzyme activity detection by the coupled pyruvate kinase/lactate dehydrogenase assay, UMP kinase assay development and optimization, overview | Staphylococcus aureus | ? | - |
? | |
additional information | enzyme activity detection by the coupled pyruvate kinase/lactate dehydrogenase assay, UMP kinase assay development and optimization, overview | Staphylococcus aureus ATCC 601055 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | analytical ultracentrifugation | Staphylococcus aureus |
Synonyms | Comment | Organism |
---|---|---|
pyrH | - |
Staphylococcus aureus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Staphylococcus aureus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Staphylococcus aureus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Staphylococcus aureus | |
ATP | - |
Staphylococcus aureus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0156 | - |
pH 7.5, 22°C | Staphylococcus aureus | N4,6-diphenylpyrimidine-2,4-diamine | |
0.025 | - |
pH 7.5, 22°C | Staphylococcus aureus | N4-ethyl-N4,6-diphenylpyrimidine-2,4-diamine | |
0.1 | - |
above, pH 7.5, 22°C | Staphylococcus aureus | N4-(4-methylphenyl)-6-phenylpyrimidine-2,4-diamine | |
0.1 | - |
above, pH 7.5, 22°C | Staphylococcus aureus | N4-(4-methoxyphenyl)-6-phenylpyrimidine-2,4-diamine | |
0.1 | - |
above, pH 7.5, 22°C | Staphylococcus aureus | N4-(3-methylphenyl)-6-phenylpyrimidine-2,4-diamine | |
4.6 | - |
pH 7.5, 22°C | Staphylococcus aureus | 1-[2-[(2-amino-6-phenylpyrimidin-4-yl)amino]phenyl]ethanone | |
32.9 | - |
pH 7.5, 22°C | Staphylococcus aureus | N4-(2-methoxyphenyl)-6-phenylpyrimidine-2,4-diamine | |
44 | - |
pH 8.5, 23°C | Staphylococcus aureus | 2-amino-8-[2-(4-methoxyphenyl)-2-oxoethoxy]-1,4,5,9-tetrahydro-6H-purin-6-one | |
51 | - |
pH 8.5, 23°C | Staphylococcus aureus | 2-amino-8-[2-(3-methylphenyl)-2-oxoethoxy]-1,4,5,9-tetrahydro-6H-purin-6-one | |
53.8 | - |
pH 7.5, 22°C | Staphylococcus aureus | N4-(2-methylphenyl)-6-phenylpyrimidine-2,4-diamine | |
64 | - |
pH 8.5, 23°C | Staphylococcus aureus | 2-amino-8-[2-(4-methylphenyl)-2-oxoethoxy]-1,4,5,9-tetrahydro-6H-purin-6-one | |
79 | - |
pH 8.5, 23°C | Staphylococcus aureus | 2-amino-8-[2-(4-fluorophenyl)-2-oxoethoxy]-1,4,5,9-tetrahydro-6H-purin-6-one | |
82 | - |
pH 8.5, 23°C | Staphylococcus aureus | 2-amino-8-[2-(4-hydroxyphenyl)-2-oxoethoxy]-1,4,5,9-tetrahydro-6H-purin-6-one | |
88 | - |
pH 8.5, 23°C | Staphylococcus aureus | 4-[[(2-amino-6-oxo-4,5,6,9-tetrahydro-1H-purin-8-yl)oxy]acetyl]benzonitrile | |
139 | - |
pH 8.5, 23°C | Staphylococcus aureus | 2-amino-8-(2-oxo-2-phenylethoxy)-1,4,5,9-tetrahydro-6H-purin-6-one | |
243 | - |
pH 8.5, 23°C | Staphylococcus aureus | 2-amino-8-[2-(2-methylphenyl)-2-oxoethoxy]-1,4,5,9-tetrahydro-6H-purin-6-one | |
254 | - |
pH 8.5, 23°C | Staphylococcus aureus | 4-[(2-amino-6-oxo-4,5,6,9-tetrahydro-1H-purin-8-yl)methyl]benzonitrile |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme is a member of the NMP kinase family | Staphylococcus aureus |
physiological function | UMP kinase is an essential enzyme found only in bacteria | Staphylococcus aureus |