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Literature summary for 2.7.4.22 extracted from

  • Marco-Marin, C.; Rubio, V.
    The site for the allosteric activator GTP of Escherichia coli UMP kinase (2009), FEBS Lett., 583, 185-189.
    View publication on PubMed
Search for more literature for 2.7.4.22

Activating Compound

Activating Compound Comment Organism Structure
5'-guanylyl-imidodiphosphate
-
 Escherichia coli
GTP
-
 Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
His-tagged version expressed in Escherichia coli BL21(DE3) Escherichia coli

Protein Variants

Protein Variants Comment Organism
D115A little detrimental effect, activated by 5'-guanylyl-imidodiphosphate within a concentration range that is roughly similar to that of wild type enzyme Escherichia coli
H96A involved in GTP activation, abolishing GTP activation Escherichia coli
N111A little detrimental effect, activated by 5'-guanylyl-imidodiphosphate within a concentration range that is roughly similar to that of wild type enzyme Escherichia coli
R103A involved in GTP activation, abolishing GTP activation Escherichia coli
R127A decreasing affinity for GTP Escherichia coli
R130A involved in GTP activation, abolishing GTP activation Escherichia coli
R92A involved in GTP activation, abolishing GTP activation Escherichia coli
S124A decreasing affinity for GTP Escherichia coli
W119A involved in GTP activation, abolishing GTP activation Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
UTP
-
 Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P0A7E9
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 R92A, H96A, and R127A exhibit increased enzyme activity Escherichia coli

Synonyms

Synonyms Comment Organism
UMP kinase
-
 Escherichia coli