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Literature summary for 2.7.4.2 extracted from
- Kinetics of phosphomevalonate kinase from Saccharomyces cerevisiae (2014), PLoS ONE, 9, e87112.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Saccharomyces cerevisiae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the enzyme is not substrate inhibited, nor feedback inhibited by farnesyl diphosphate at concentrations up to 10 mM | Saccharomyces cerevisiae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0743 | - |
ATP | at pH 7.2 and 37°C | Saccharomyces cerevisiae |  |
| 0.0983 | - |
ATP | at pH 7.2 and 30°C | Saccharomyces cerevisiae | |
| 0.88 | - |
(R)-5-phosphomevalonate | at pH 7.2 and 37°C | Saccharomyces cerevisiae | |
| 0.885 | - |
(R)-5-phosphomevalonate | at pH 7.2 and 30°C | Saccharomyces cerevisiae | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | dependent on, maximal activity achieved at concentrations of 10 mM or greater | Saccharomyces cerevisiae | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + (R)-5-phosphomevalonate | Saccharomyces cerevisiae | - |
ADP + (R)-5-diphosphomevalonate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | P24521 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA resin column chromatography | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + (R)-5-phosphomevalonate | - |
Saccharomyces cerevisiae | ADP + (R)-5-diphosphomevalonate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PMK | - |
Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
- |
Saccharomyces cerevisiae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 8 | the enzyme activity drops off below pH 6.5 and above pH 8.0 | Saccharomyces cerevisiae |
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Release 2023.1 (January 2023)
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