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Literature summary for 2.7.4.2 extracted from

  • Herdendorf, T.J.; Miziorko, H.M.
    Phosphomevalonate kinase: functional investigation of the recombinant human enzyme (2006), Biochemistry, 45, 3235-3242.
    View publication on PubMed
Search for more literature for 2.7.4.2

Cloned(Commentary)

Cloned (Comment) Organism
gene PMVK, DNA and amino acid sequence determination and analysis, functional expression of soluble His-tagged wild-type enzyme and of His-tagged mutants in Escherichia coli strains JM109 and BL21(DE3) Homo sapiens

Protein Variants

Protein Variants Comment Organism
D23N site-directed mutagenesis, the mutant shows highly reduced activity in both forward and reverse reactions compared to the wild-type enzyme Homo sapiens
K17M site-directed mutagenesis, the mutant shows reduced activity in both forward and reverse reactions compared to the wild-type enzyme Homo sapiens
K19M site-directed mutagenesis, the mutant shows highly reduced activity in both forward and reverse reactions compared to the wild-type enzyme Homo sapiens
K22M site-directed mutagenesis, the mutant shows a 10000fold reduced activity in both forward and reverse reactions compared to the wild-type enzyme, almost inactive mutant Homo sapiens
R18Q site-directed mutagenesis, the mutant shows highly reduced activity in both forward and reverse reactions compared to the wild-type enzyme Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information steady-state kinetics Homo sapiens
0.034
-
 (R)-mevalonate 5-phosphate pH 7.0, 30°C, recombinant wild-type enzyme Homo sapiens
0.041
-
 (R)-mevalonate 5-diphosphate pH 7.0, 30°C, recombinant wild-type enzyme Homo sapiens
0.047
-
 ADP pH 7.0, 30°C, recombinant wild-type enzyme Homo sapiens
0.047
-
 (R)-mevalonate 5-diphosphate pH 7.0, 30°C, recombinant mutant R18Q Homo sapiens
0.065
-
 ADP pH 7.0, 30°C, recombinant mutant R18Q Homo sapiens
0.079
-
 ADP pH 7.0, 30°C, recombinant mutant D23N Homo sapiens
0.079
-
 (R)-mevalonate 5-diphosphate pH 7.0, 30°C, recombinant mutant K19M Homo sapiens
0.107
-
 ATP pH 7.0, 30°C, recombinant wild-type enzyme Homo sapiens
0.107
-
 ATP ATP in form of MgATP2- Homo sapiens
0.116
-
 (R)-mevalonate 5-phosphate pH 7.0, 30°C, recombinant mutant D23N Homo sapiens
0.123
-
 (R)-mevalonate 5-phosphate pH 7.0, 30°C, recombinant mutant R18Q Homo sapiens
0.131
-
 ADP pH 7.0, 30°C, recombinant mutant K19M Homo sapiens
0.138
-
 (R)-mevalonate 5-diphosphate pH 7.0, 30°C, recombinant mutant D23N Homo sapiens
0.176
-
 (R)-mevalonate 5-phosphate pH 7.0, 30°C, recombinant mutant K17M Homo sapiens
0.187
-
 (R)-mevalonate 5-diphosphate pH 7.0, 30°C, recombinant mutant K17M Homo sapiens
0.23
-
 (R)-mevalonate 5-phosphate pH 7.0, 30°C, recombinant mutant K19M Homo sapiens
0.286
-
 ADP pH 7.0, 30°C, recombinant mutant K17M Homo sapiens
0.536
-
 ATP pH 7.0, 30°C, recombinant mutant R18Q Homo sapiens
0.536
-
 ATP ATP in form of MgATP2- Homo sapiens
0.556
-
 ATP pH 7.0, 30°C, recombinant mutant K17M Homo sapiens
0.556
-
 ATP ATP in form of MgATP2- Homo sapiens
0.815
-
 ATP pH 7.0, 30°C, recombinant mutant D23N Homo sapiens
0.815
-
 ATP ATP in form of MgATP2- Homo sapiens
1.312
-
 ATP pH 7.0, 30°C, recombinant mutant K19M Homo sapiens
1.312
-
 ATP ATP in form of MgATP2- Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ as MgATP2- Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
22900
-
 recombinant His-tagged wild-type enzyme, gel filtration Homo sapiens
24200
-
 1 * 24200, sequence calculation Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + (R)-mevalonate 5-phosphate Homo sapiens the enzyme catalyzes a key step in isoprenoid/sterol biosynthesis ADP + (R)-mevalonate 5-diphosphate
-
 r

Organism

Organism UniProt Comment Textmining
Homo sapiens Q15126 gene PMVK
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli by nickel affinity chromatography Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
ATP + (R)-5-phosphomevalonate = ADP + (R)-5-diphosphomevalonate residue R22 is important for catalysis Homo sapiens
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (R)-5-phosphomevalonate
-
 Homo sapiens ADP + (R)-5-diphosphomevalonate
-
 ?
ATP + (R)-mevalonate 5-phosphate
-
 Homo sapiens ADP + (R)-mevalonate 5-diphosphate
-
 r
ATP + (R)-mevalonate 5-phosphate the enzyme catalyzes a key step in isoprenoid/sterol biosynthesis Homo sapiens ADP + (R)-mevalonate 5-diphosphate
-
 r

Subunits

Subunits Comment Organism
monomer 1 * 24200, sequence calculation Homo sapiens
More homology modeling for structure analysis, the model conforms to a nucleoside monophosphate kinase family fold, overview Homo sapiens

Synonyms

Synonyms Comment Organism
More the enzyme belongs to the nucleoside monophosphate, NMP kinase family Homo sapiens
PMK
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ADP
-
 Homo sapiens
ATP as MgATP2-, the enzyme possesses an N-terminal basic residue rich sequence, a possible Walker A ATP binding motif Homo sapiens