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Literature summary for 2.7.4.2 extracted from

  • Doun, S.S.; Burgner, J.W.2nd.; Briggs, S.D.; Rodwell, V.W.
    Enterococcus faecalis phosphomevalonate kinase (2005), Protein Sci., 14, 1134-1139.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.4.2

Application

Application Comment Organism
medicine the enzymes of the mevalonate pathway of isopentenyl diphosphate biosynthesis represent potential for addressing a pressing human health concern, the development of antibiotics against resistent strains of the Gram-positive streptococci Enterococcus faecalis

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21(DE3) Enterococcus faecalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.17
-
 ATP
-
 Enterococcus faecalis
0.19
-
 (R,S)-5-phosphomevalonate
-
 Enterococcus faecalis

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 10 mM cannot replace Mn2+ or Mg2+ Enterococcus faecalis
Mn2+ preferred cation to activate the enzyme, optimal concentration about 4 mM Enterococcus faecalis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40500
-
 2 * 48000, SDS-PAGE, analytical ultracentrifugation, 2 * 40500, amino acid sequence Enterococcus faecalis
48000
-
 2 * 48000, SDS-PAGE, analytical ultracentrifugation, 2 * 40500, amino acid sequence Enterococcus faecalis
76000
-
 analytical ultracentrifugation Enterococcus faecalis
81000
-
 calculated from amino acid sequence Enterococcus faecalis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + (R)-5-phosphomevalonate Enterococcus faecalis mevalonate pathway of isopentenyl diphosphate biosynthesis ADP + (R)-5-diphosphomevalonate
-
 ?
ATP + (R)-5-phosphomevalonate Enterococcus faecalis 41 mevalonate pathway of isopentenyl diphosphate biosynthesis ADP + (R)-5-diphosphomevalonate
-
 ?

Organism

Organism UniProt Comment Textmining
Enterococcus faecalis
-
-
-
Enterococcus faecalis 41
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Enterococcus faecalis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.9
-
-
 Enterococcus faecalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (R)-5-phosphomevalonate mevalonate pathway of isopentenyl diphosphate biosynthesis Enterococcus faecalis ADP + (R)-5-diphosphomevalonate
-
 ?
ATP + (R)-5-phosphomevalonate mevalonate pathway of isopentenyl diphosphate biosynthesis Enterococcus faecalis 41 ADP + (R)-5-diphosphomevalonate
-
 ?
ATP + (R,S)-5-phosphomevalonate
-
 Enterococcus faecalis ADP + (R,S)-5-diphosphomevalonate
-
 ?
ATP + (R,S)-5-phosphomevalonate
-
 Enterococcus faecalis 41 ADP + (R,S)-5-diphosphomevalonate
-
 ?
CTP + (R,S)-5-phosphomevalonate 0.4% activity Enterococcus faecalis CDP + (R,S)-5-diphosphomevalonate
-
 ?
CTP + (R,S)-5-phosphomevalonate 0.4% activity Enterococcus faecalis 41 CDP + (R,S)-5-diphosphomevalonate
-
 ?
GTP + (R,S)-5-phosphomevalonate 3.6% activity Enterococcus faecalis GDP + (R,S)-5-diphosphomevalonate
-
 ?
GTP + (R,S)-5-phosphomevalonate 3.6% activity Enterococcus faecalis 41 GDP + (R,S)-5-diphosphomevalonate
-
 ?
TTP + (R,S)-5-phosphomevalonate 1.6% activity Enterococcus faecalis TDP + (R,S)-5-diphosphomevalonate
-
 ?
TTP + (R,S)-5-phosphomevalonate 1.6% activity Enterococcus faecalis 41 TDP + (R,S)-5-diphosphomevalonate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 48000, SDS-PAGE, analytical ultracentrifugation, 2 * 40500, amino acid sequence Enterococcus faecalis

Synonyms

Synonyms Comment Organism
phosphomevalonate kinase
-
 Enterococcus faecalis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Enterococcus faecalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Enterococcus faecalis