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Literature summary for 2.7.4.2 extracted from
- Phosphomevalonate kinase from pig liver (1985), Methods Enzymol., 110, 78-85.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.056 | - |
ATP | pH 7.5, 30°C | Sus scrofa |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | can partially replace Mg2+ in activation | Sus scrofa | |
| Mg2+ | divalent cation required, Mg2+ most efficient | Sus scrofa | |
| Mn2+ | can partially replace Mg2+ in activation | Sus scrofa | |
| Zn2+ | can partially replace Mg2+ in activation | Sus scrofa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 21000 | - |
1 * 21000, SDS-PAGE | Sus scrofa |
| 22000 | - |
- |
Sus scrofa |
| 22500 | - |
- |
Sus scrofa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sus scrofa | - |
- |
- |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| very unstable in absence of thiol compounds | Sus scrofa |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate, DEAE-cellulose, BioGel P-150, hydroxylapatite, Blue Dextran-Sepharose | Sus scrofa |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| ATP + (R)-5-phosphomevalonate = ADP + (R)-5-diphosphomevalonate | ordered mechanism | Sus scrofa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Sus scrofa | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 69.1 | - |
- |
Sus scrofa |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 10 mM Tris-HCl, pH 7.5, 1 mM dithiothreitol, 50% glycerol, 15 months, 20% loss of acctivity | Sus scrofa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 5-phosphomevalonate | (3R)-phosphomevalonate | Sus scrofa | ADP + 5-diphosphomevalonate | (3R)-diphosphomevalonate | r | |
| ATP + 5-phosphomevalonate | absolute specificity for ATP | Sus scrofa | ADP + 5-diphosphomevalonate | (3R)-diphosphomevalonate | r | |
| ATP + 5-phosphomevalonate | the equilibrium constant is near unity at pH 7.5 and 30°C | Sus scrofa | ADP + 5-diphosphomevalonate | (3R)-diphosphomevalonate | r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 21000, SDS-PAGE | Sus scrofa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Sus scrofa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 9.5 | - |
Sus scrofa |
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Release 2023.1 (January 2023)
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