Protein Variants | Comment | Organism |
---|---|---|
additional information | enzyme knockdown in MCF-7 cells using a CMPK shRNA lentivirus | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
nucleus | localization of enzyme CMPK at DNA damage sites, co-localization and complex formation with Tip60 and ribonucleotide reductase | Homo sapiens | 5634 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + CMP | Homo sapiens | - |
ADP + CDP | - |
? | |
ATP + UMP | Homo sapiens | - |
ADP + UDP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P30085 | gene CMPK1 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
breast adenocarcinoma cell | - |
Homo sapiens | - |
MCF-7 cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + CMP | - |
Homo sapiens | ADP + CDP | - |
? | |
ATP + CMP | preferred substrate | Homo sapiens | ADP + CDP | - |
? | |
ATP + dCMP | - |
Homo sapiens | ADP + dCDP | - |
? | |
ATP + dUMP | - |
Homo sapiens | ADP + dUDP | - |
? | |
ATP + UMP | - |
Homo sapiens | ADP + UDP | - |
? | |
ATP + UMP | preferred substrate | Homo sapiens | ADP + UDP | - |
? | |
additional information | CMP and UMP are the preferred substrates for purified CMPK, and it has lower affinity to dCMP and dUMP | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CMP kinase | - |
Homo sapiens |
CMP/UMP kinase | - |
Homo sapiens |
CMPK | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
malfunction | knockdown of CMPK delays DNA repair during recovery from UV damage in serum-deprived cells but not in the cells without serum deprivation. Exogenous supply of cytidine or deoxycytidine facilitates DNA repair dependent on CMPK in serum-deprived cells. But CMPK knockdown does not affect the steady state level of dCTP in serum-deprived cells. Re-expression of wild-type but not N-terminus deleted CMPK restores the efficiency of DNA repair in CMPK knockdown cells | Homo sapiens |
additional information | the N-terminal region of CMPK is essential for the complex formation and recruitment to DNA damage sites | Homo sapiens |
physiological function | the enzyme enzyme catalyzes CDP formation in DNA repair, the synthesis of dCDP or CDP determines the rate of repair. The N-terminal 32-amino-acid of enzyme CMPK is required for its recruitment to DNA damage sites in a Tip60-dependent manner, complex of CMPK/RNR/Tip60 and the recruitment to the sites of DNA damage, overview. Site-specific dCDP formation via CMPK provides a means to facilitate DNA repair in serum-deprived cells, analysis of functional contribution of CMPK to dCTP pool and the rate of DNA compared in MCF-7 cells in serum-containing and serum-deprived conditions | Homo sapiens |