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Literature summary for 2.7.4.14 extracted from

  • Topalis, D.; Kumamoto, H.; Amaya Velasco, M.F.; Dugue, L.; Haouz, A.; Alexandre, J.A.; Gallois-Montbrun, S.; Alzari, P.M.; Pochet, S.; Agrofoglio, L.A.; Deville-Bonne, D.
    Nucleotide binding to human UMP-CMP kinase using fluorescent derivatives - a screening based on affinity for the UMP-CMP binding site (2007), FEBS J., 274, 3704-3714.
    View publication on PubMed
Search for more literature for 2.7.4.14

Application

Application Comment Organism
medicine screening of acyclic phosphonate analogs to find antimetabolites for antivirus and anticancer therapies Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli, wild type and selenomethionine labelled protein Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
of the selenomethionine labelled protein in complex with UMP, CMP, ADP, AMP-PCP, cidofovor and P1-(5’-adenosyl) P5-(5’-uridyl) pentaphosphate Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
5-Br-uracil vinylphosphonate
-
 Homo sapiens
5-Cl-uracil vinylphosphonate
-
 Homo sapiens
5-F-Phe-uracil vinylphosphonate
-
 Homo sapiens
5-Phe-S-uracil vinylphosphonate
-
 Homo sapiens
5-Phe-uracil vinylphosphonate
-
 Homo sapiens
uracil vinylphosphonate
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.07
-
 CMP
-
 Homo sapiens
0.13
-
 UMP
-
 Homo sapiens
1
-
 Cidofovir
-
 Homo sapiens
1.1
-
 dCMP
-
 Homo sapiens
1.3
-
 dUMP
-
 Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P30085
-
-

Purification (Commentary)

Purification (Comment) Organism
of the recombinant protein Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (S)-1-(3-hydroxy-2-phosphonylmethoxypropyl) cytosine Cidofovir Homo sapiens ADP + (S)-1-(3-hydroxy-2-phosphonylmethoxypropyl) cytosine
-
 ?
ATP + CMP
-
 Homo sapiens ADP + CDP
-
 ?
ATP + dCMP
-
 Homo sapiens ADP + dCDP
-
 ?
ATP + dUMP no activity with CDP, dTMP, AMP, GMP Homo sapiens ADP + dUDP
-
 ?
ATP + UMP
-
 Homo sapiens ADP + UDP
-
 ?

Synonyms

Synonyms Comment Organism
cytidylate kinase
-
 Homo sapiens
UCK
-
 Homo sapiens
UMP-CMP kinase
-
 Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.016
-
 5-Cl-uracil vinylphosphonate
-
 Homo sapiens
0.017
-
 5-Br-uracil vinylphosphonate
-
 Homo sapiens
0.028
-
 5-Phe-S-uracil vinylphosphonate
-
 Homo sapiens
0.7
-
 uracil vinylphosphonate
-
 Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.03
-
-
 Homo sapiens 5-Cl-uracil vinylphosphonate
0.035
-
-
 Homo sapiens 5-Br-uracil vinylphosphonate
0.05
-
-
 Homo sapiens 5-Phe-S-uracil vinylphosphonate
0.12
-
 amplitude 50% Homo sapiens 5-Phe-S-uracil vinylphosphonate
0.23
-
-
 Homo sapiens 5-F-Phe-uracil vinylphosphonate
1.2
-
-
 Homo sapiens uracil vinylphosphonate