Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | activates | Saccharomyces cerevisiae | |
dithiothreitol | 1 mM, activates | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CMP | competitive inhibition of UMP phosphorylation | Saccharomyces cerevisiae | |
dCMP | competitive inhibition of UMP phosphorylation | Saccharomyces cerevisiae | |
HgCl2 | 0.1 mM, 88% inhibition | Saccharomyces cerevisiae | |
NEM | 1.0 mM; 41% inhibition | Saccharomyces cerevisiae | |
PCMB | 0.1 mM, 69% loss of activity | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.052 | - |
UMP | pH 7.5, 25°C | Saccharomyces cerevisiae | |
0.071 | - |
CMP | pH 7.5, 25°C | Saccharomyces cerevisiae | |
0.54 | - |
dCMP | pH 7.5, 25°C | Saccharomyces cerevisiae | |
8.5 | - |
dUMP | pH 7.5, 25°C | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | divalent cation required, Mn2+ can substitute for Mg2+ | Saccharomyces cerevisiae | |
Mg2+ | divalent cation required, Mg2+ is most effective | Saccharomyces cerevisiae | |
Mn2+ | divalent cation required, Mn2+ can substitute for Mg2+ | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26000 | - |
gel filtration | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
11.1 | - |
- |
Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + CMP | - |
Saccharomyces cerevisiae | ADP + CDP | - |
? | |
ATP + dCMP | - |
Saccharomyces cerevisiae | ADP + dCDP | - |
? | |
ATP + dUMP | - |
Saccharomyces cerevisiae | ADP + dUDP | - |
? | |
ATP + UMP | - |
Saccharomyces cerevisiae | ADP + UDP | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | - |
at pH 7 the enzyme is most stable when kept at 4°C | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
reaction with UMP and ATP | Saccharomyces cerevisiae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 9 | pH 4.0: about 40% of maximal activity, pH 9.0: about 65% of maximal activity, reaction with UMP and ATP | Saccharomyces cerevisiae |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | - |
at pH 7.0, the enzyme is most stable when kept at 4°C | Saccharomyces cerevisiae |