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Literature summary for 2.7.4.10 extracted from

  • Ulschmid, J.K.; Rahlfs, S.; Schirmer, R.H.; Becker, K.
    Adenylate kinase and GTP:AMP phosphotransferase of the malarial parasite Plasmodium falciparum. Central players in cellular energy metabolism (2004), Mol. Biochem. Parasitol., 136, 211-220.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli strain M15 Plasmodium falciparum

Inhibitors

Inhibitors Comment Organism Structure
Atractyloside
-
 Plasmodium falciparum
guanosine(5')pentaphospho(5')adenosine
-
 Plasmodium falciparum
additional information not inhibitory at concentrations up to 1 mM: chloroquine, primaquine, artemisinine, mefloquine Plasmodium falciparum
P1,P5-di(adenosine-5')pentaphosphate
-
 Plasmodium falciparum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 ATP Km for ATP more than 5 mM Plasmodium falciparum
0.13
-
 GTP
-
 Plasmodium falciparum
0.21
-
 AMP
-
 Plasmodium falciparum

Metals/Ions

Metals/Ions Comment Organism Structure
Fe 0.3 mol Fe/mol protein Plasmodium falciparum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26700
-
 calculated without His-tag Plasmodium falciparum

Organism

Organism UniProt Comment Textmining
Plasmodium falciparum Q964H2 strains HB3 and Dd2
-

Purification (Commentary)

Purification (Comment) Organism
-
 Plasmodium falciparum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
100
-
 25°C Plasmodium falciparum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + AMP
-
 Plasmodium falciparum 2 ADP
-
 ?
CTP + AMP
-
 Plasmodium falciparum CDP + ADP
-
 ?
GTP + AMP
-
 Plasmodium falciparum GDP + ADP
-
 ?
GTP + IMP
-
 Plasmodium falciparum GDP + IDP
-
 ?
ITP + AMP
-
 Plasmodium falciparum IDP + ADP
-
 ?
additional information GTP + GMP/CMP/UMP activity below 1%, ATP + AMP activity below 1% Plasmodium falciparum ?
-
 ?
UTP + AMP
-
 Plasmodium falciparum UDP + ADP
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Plasmodium falciparum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00014
-
 guanosine(5')pentaphospho(5')adenosine 25°C, pH 6 Plasmodium falciparum
0.00021
-
 guanosine(5')pentaphospho(5')adenosine 25°C, pH 7.4 Plasmodium falciparum
0.0114
-
 P1,P5-di(adenosine-5')pentaphosphate 25°C, pH 6 Plasmodium falciparum