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Literature summary for 2.7.4.10 extracted from
- Purification and properties of nucleoside triphosphate-adenosine monophosphate transphosphorylase from beef heart mitochondria (1970), Biochemistry, 9, 2462-2470.
General Stability
| General Stability | Organism |
|---|---|
| ammonium sulfate or substrates enhance stability | Bos taurus |
| freeze-thawing, dilutions, or low ionic strength decreases activity rapidly | Bos taurus |
| stability increases during purification | Bos taurus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | reverse reaction, kinetics | Bos taurus |  |
| AMP | free form, Mg2+ reverses | Bos taurus | |
| ATP | - |
Bos taurus | |
| GDP | reverse reaction, kinetics | Bos taurus | |
| GTP | kinetics | Bos taurus | |
| Mg2+ | weak, above 4 mM, activates below | Bos taurus | |
| additional information | no inhibition by EDTA, GSH, cysteamine or high phosphate concentrations | Bos taurus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0012 | - |
GDP | pH 8.0, with ADP | Bos taurus | |
| 0.033 | - |
AMP | - |
Bos taurus | |
| 0.056 | - |
GTP | pH 8.0, with AMP | Bos taurus | |
| 0.29 | - |
ADP | pH 8.0, with GDP | Bos taurus | |
| 0.63 | - |
ITP | pH 8.0, with AMP | Bos taurus | |
| 1 | - |
ATP | pH 8.0, with of AMP | Bos taurus | |
| 7.4 | - |
UTP | pH 8.0, with of AMP | Bos taurus | |
| 9.1 | - |
CTP | pH 8.0, with of AMP | Bos taurus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | matrix | Bos taurus | 5739 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activation, 50% as effective as Mg2+ | Bos taurus | |
| Mg2+ | activation, 4 mM, as good as Mn2+, slightly inhibitory above 4 mM | Bos taurus | |
| Mn2+ | activation, as good as Mg2+ | Bos taurus | |
| additional information | no activation by EDTA, cysteamine, GSH or high phosphate concentrations | Bos taurus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 52000 | - |
gel filtration | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bos taurus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| nucleoside triphosphate + AMP = nucleoside diphosphate + ADP | mechanism | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| heart | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 135 | - |
- |
Bos taurus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0°C, partially purified preparation, several weeks | Bos taurus |
| room temperature, 1 week | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + AMP | poor substrate, highly specific for AMP, phosphorylation at about 5% the rate of ITP | Bos taurus | 2 ADP | - |
r | |
| CTP + AMP | highly specific for AMP, phosphorylation at about 11% the rate of ITP | Bos taurus | CDP + ADP | - |
r | |
| GTP + AMP | highly specific for AMP and ADP, in reverse reaction | Bos taurus | GDP + ADP | via nucleotide-enzyme complex, no phosphorylated enzyme intermediate | r | |
| GTP + AMP | phosphorylation at about 90% the rate of ITP | Bos taurus | GDP + ADP | via nucleotide-enzyme complex, no phosphorylated enzyme intermediate | r | |
| ITP + AMP | best substrate | Bos taurus | IDP + ADP | via nucleotide-enzyme complex, no phosphorylated enzyme intermediate | r | |
| ITP + AMP | highly specific for AMP and ADP in reverse reaction | Bos taurus | IDP + ADP | via nucleotide-enzyme complex, no phosphorylated enzyme intermediate | r | |
| ITP + AMP | no acceptor substrates are 3'-GMP | Bos taurus | IDP + ADP | via nucleotide-enzyme complex, no phosphorylated enzyme intermediate | r | |
| ITP + AMP | not: IMP, CMP, UMP | Bos taurus | IDP + ADP | via nucleotide-enzyme complex, no phosphorylated enzyme intermediate | r | |
| UTP + AMP | highly specific for AMP, phosphorylation at about 19% the rate of ITP | Bos taurus | UDP + ADP | - |
r | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
35% ammonium sulfate, AMP or other substrates enhance thermal stability | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Bos taurus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9.7 | about half-maximal activity at pH 7 and 9.7 | Bos taurus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.73 | - |
ATP | pH 8.5, competitive with AMP | Bos taurus | |
| 0.74 | - |
ATP | pH 8.5, competitive with GTP | Bos taurus | |
| 0.77 | - |
GDP | pH 8.5 | Bos taurus | |
| 0.8 | - |
GTP | pH 8.5 | Bos taurus | |
| 0.9 | - |
ADP | pH 8.5 | Bos taurus | |
| 1.6 | - |
AMP | pH 8.5 | Bos taurus | |
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