Literature summary for 2.7.4.1 extracted from

McMahon, K.D.; Dojka, M.A.; Pace, N.R.; Jenkins, D.; Keasling, J.D.
Polyphosphate kinase from activated sludge performing enhanced biological phosphorus removal (2002), Appl. Environ. Microbiol., 68, 4971-4978.

Search for more literature for 2.7.4.1

Cloned(Commentary)

Cloned (Comment)	Organism
expression of type I polyphosphate kinase in Escherichia coli	Rhodocyclus sp.

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.2	-	ATP	pH 7.2, room temperature, recombinant polyphosphate kinase	Rhodocyclus sp.

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	recombinant polyphosphate kinase, maximal activity at 10 mM	Rhodocyclus sp.

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
75000	-	recombinant polyphosphate kinase, gel filtration	Rhodocyclus sp.

Organism

Organism	UniProt	Comment	Textmining
Rhodocyclus sp.	Q8KT80	Rhodocyclus-like beta-Proteobacteria enriched in activating sludge carrying out enhanced biological phosphorus removal	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.034	-	recombinant polyphosphate kinase	Rhodocyclus sp.

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + (phosphate)n	very slow reverse reaction	Rhodocyclus sp.	ADP + (phosphate)n+1	-	r

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	recombinant polyphosphate kinase	Rhodocyclus sp.

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.4	-	recombinant polyphosphate kinase	Rhodocyclus sp.

pH Range

pH Minimum	pH Maximum	Comment	Organism
5	9	recombinant polyphosphate kinase	Rhodocyclus sp.

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Release 2023.1 (January 2023)