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Literature summary for 2.7.3.9 extracted from
- The monomer/dimer transition of enzyme I of the Escherichia coli phosphotransferase system (2006), J. Biol. Chem., 281, 17570-17578.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| in overproducing transformants | Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| H189Q | site directed mutagenesis | Escherichia coli |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | induces small conformational changes, large conformational changes with Mg2+ and phosphoenolpyruvate, swivelling mechanism, dimerization of the enzyme depends on its ligands | Escherichia coli |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P08839 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| from overproducing transformants | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphoenolpyruvate + protein histidine | - |
Escherichia coli | pyruvate + protein N-pros-phosphohistidine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | homodimers accept phosphoryl group from phosphoenolpyruvate, monomer does not | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| enzyme I of the phosphotransferase system | - |
Escherichia coli |
| PEP:sugar phosphotransferase system enzyme I | - |
Escherichia coli |
| phosphoenolpyruvate-protein phosphotransferase | - |
Escherichia coli |
| phosphoenolpyruvate:protein-L-histidine N-pros-phosphotransferase | - |
Escherichia coli |
| phosphotransferase system enzyme I | - |
Escherichia coli |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 15 | 35 | dimerization of the enzyme depends on the temperature | Escherichia coli |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 7.5 | dimerization of the enzyme depends on pH | Escherichia coli |
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