Cloned (Comment) | Organism |
---|---|
in overproducing transformants | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
H189Q | site directed mutagenesis | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | induces small conformational changes, large conformational changes with Mg2+ and phosphoenolpyruvate, swivelling mechanism, dimerization of the enzyme depends on its ligands | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P08839 | - |
- |
Purification (Comment) | Organism |
---|---|
from overproducing transformants | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + protein histidine | - |
Escherichia coli | pyruvate + protein N-pros-phosphohistidine | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | homodimers accept phosphoryl group from phosphoenolpyruvate, monomer does not | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
enzyme I of the phosphotransferase system | - |
Escherichia coli |
PEP:sugar phosphotransferase system enzyme I | - |
Escherichia coli |
phosphoenolpyruvate-protein phosphotransferase | - |
Escherichia coli |
phosphoenolpyruvate:protein-L-histidine N-pros-phosphotransferase | - |
Escherichia coli |
phosphotransferase system enzyme I | - |
Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | 35 | dimerization of the enzyme depends on the temperature | Escherichia coli |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7.5 | dimerization of the enzyme depends on pH | Escherichia coli |