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Literature summary for 2.7.3.9 extracted from
- Synthesis of HPr(Ser-P)(His-P) by enzyme I of the phosphoenolpyruvate: sugar phosphotransferase system of Streptococcus salivarius (2006), Biochemistry, 45, 6692-6702.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Streptococcus salivarius |
| expression in Escherichia coli | Bacillus subtilis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 12 | - |
protein histidine | at pH 6,1 | Streptococcus salivarius |  |
| 20 | - |
protein histidine | at pH 7,0 | Streptococcus salivarius | |
| 39 | - |
protein histidine | at pH 7,9 | Streptococcus salivarius | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 130000 | - |
gel filtration | Bacillus subtilis |
| 130000 | - |
dimer, gel filtration | Streptococcus salivarius |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | P08838 | - |
- |
| Streptococcus salivarius | P30299 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| of the recombinant protein | Streptococcus salivarius |
| of the recombinant protein | Bacillus subtilis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 145 | 400 | at 37°C | Streptococcus salivarius |
Storage Stability
| Storage Stability | Organism |
|---|---|
| several months at -80°C, up to 1 h at 37°C | Streptococcus salivarius |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphoenolpyruvate + protein histidine | HPr, heat stable histidine containing protein, HPr(Ser-P) heat stable histidine containing protein phosphorylated at serine 46, HPr(His-P) heat stable histidine containing protein phosphorylated at histidine 15, HPr-P2 heat stable histidine containing protein doubly phosphorylated, HPr(S46D) heat stable histidine containing protein in which serine is replaced by asparate | Streptococcus salivarius | pyruvate + protein N-pros-phosphohistidine | - |
? | |
| phosphoenolpyruvate + protein histidine | HPr, heat stable histidine containing protein, HPr(Ser-P) heat stable histidine containing protein phosphorylated at serine 46, HPr(His-P) heat stable histidine containing protein phosphorylated at histidine 15, HPr-P2 heat stable histidine containing protein doubly phosphorylated, HPr(S46D) heat stable histidine containing protein in which serine is replaced by asparate | Bacillus subtilis | pyruvate + protein N-pros-phosphohistidine | - |
? | |
| phosphoenolpyruvate + protein histidine | - |
Streptococcus salivarius | pyruvate + protein Npi-phospho-L-histidine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | - |
Streptococcus salivarius |
| dimer | - |
Bacillus subtilis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| enzyme I of the phosphotransferase system | - |
Streptococcus salivarius |
| enzyme I of the phosphotransferase system | - |
Bacillus subtilis |
| PEP:sugar phosphotransferase system enzyme I | - |
Streptococcus salivarius |
| PEP:sugar phosphotransferase system enzyme I | - |
Bacillus subtilis |
| phosphoenolpyruvate-protein phosphotransferase | - |
Streptococcus salivarius |
| phosphoenolpyruvate-protein phosphotransferase | - |
Bacillus subtilis |
| phosphoenolpyruvate:protein-L-histidine N-pros-phosphotransferase | - |
Streptococcus salivarius |
| phosphoenolpyruvate:protein-L-histidine N-pros-phosphotransferase | - |
Bacillus subtilis |
| phosphotransferase system enzyme I | - |
Streptococcus salivarius |
| phosphotransferase system enzyme I | - |
Bacillus subtilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.9 | - |
HPr(Ser-P) as substrate | Streptococcus salivarius |
| 6.1 | - |
HPr(S46D) as substrate | Streptococcus salivarius |
| 6.1 | - |
HPr(Ser-P) as substrate | Bacillus subtilis |
| 7 | - |
HPr as substrate | Streptococcus salivarius |
| 7.9 | - |
HPr as substrate | Bacillus subtilis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 7.5 | HPr(Ser-P) and HPr(S46D) as substrates | Streptococcus salivarius |
| 5.9 | 8.5 | HPr as substrate | Streptococcus salivarius |
| 5.9 | 7.5 | HPr(Ser-P) as substrate | Bacillus subtilis |
| 6.5 | 8.5 | HPr as substrate | Bacillus subtilis |
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