Protein Variants | Comment | Organism |
---|---|---|
K193G | turnover number is 68% of the wild-type value, KM-value for L-arginine is 4.1fold higher than wild-type value, Km-value for ATP is 2.2fold higher than wild-type value | Crassostrea sp. |
K194R | turnover number is 85% of the wild-type value, KM-value for L-arginine is 4.5fold higher than wild-type value, Km-value for ATP is 1.3fold higher than wild-type value | Crassostrea sp. |
N62D | turnover number is 88% of the wild-type value, KM-value for L-arginine is 1.1fold lower than wild-type value, Km-value for ATP is 1.2fold higher than wild-type value | Crassostrea sp. |
N62D/K193R | turnover number is 89% of the wild-type value, KM-value for L-arginine is 2.3fold higher than wild-type value, Km-value for ATP is 1.6fold higher than wild-type value | Crassostrea sp. |
N62G | turnover number is 53% of the wild-type value, KM-value for L-arginine is identical to wild-type value, Km-value for ATP is 1.2fold lower than wild-type value | Crassostrea sp. |
N62G/K193G | turnover number is 51% of the wild-type value, KM-value for L-arginine is 4.1fold higher than wild-type value, Km-value for ATP is 3.9fold higher than wild-type value | Crassostrea sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.31 | - |
L-arginine | pH 7.9, 25°C, mutant enzyme N62D | Crassostrea sp. | |
0.35 | - |
L-arginine | pH 7.9, 25°C, mutant enzyme N62G | Crassostrea sp. | |
0.35 | - |
L-arginine | pH 7.9, 25°C, wild-type enzyme | Crassostrea sp. | |
0.81 | - |
L-arginine | pH 7.9, 25°C, mutant enzyme N62D/K193R | Crassostrea sp. | |
0.82 | - |
ATP | pH 7.9, 25°C, mutant enzyme N62G | Crassostrea sp. | |
0.97 | - |
ATP | pH 7.9, 25°C, wild-type enzyme | Crassostrea sp. | |
1.17 | - |
ATP | pH 7.9, 25°C, mutant enzyme N62D | Crassostrea sp. | |
1.25 | - |
ATP | pH 7.9, 25°C, mutant enzyme K193R | Crassostrea sp. | |
1.44 | - |
L-arginine | pH 7.9, 25°C, mutant enzyme N62G/K193G | Crassostrea sp. | |
1.45 | - |
L-arginine | pH 7.9, 25°C, mutant enzyme K193G | Crassostrea sp. | |
1.57 | - |
L-arginine | pH 7.9, 25°C, mutant enzyme K193R | Crassostrea sp. | |
2.17 | - |
ATP | pH 7.9, 25°C, mutant enzyme K193G | Crassostrea sp. | |
2.54 | - |
ATP | pH 7.9, 25°C, mutant enzyme N62D/K193R | Crassostrea sp. | |
3.7 | 5 | ATP | pH 7.9, 25°C, mutant enzyme N62G/K193G | Crassostrea sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Crassostrea sp. | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-arginine | key enzyme in invertebrate energy metabolism | Crassostrea sp. | ADP + Nomega-phosphono-L-arginine | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
24.4 | - |
ATP | pH 7.9, 25°C, mutant enzyme N62G/K193G | Crassostrea sp. | |
24.4 | - |
L-arginine | pH 7.9, 25°C, mutant enzyme N62G/K193G | Crassostrea sp. | |
25.3 | - |
ATP | pH 7.9, 25°C, mutant enzyme N62G | Crassostrea sp. | |
25.3 | - |
L-arginine | pH 7.9, 25°C, mutant enzyme N62G | Crassostrea sp. | |
32.4 | - |
ATP | pH 7.9, 25°C, mutant enzyme K193G | Crassostrea sp. | |
32.4 | - |
L-arginine | pH 7.9, 25°C, mutant enzyme K193G | Crassostrea sp. | |
40.8 | - |
ATP | pH 7.9, 25°C, mutant enzyme K193R | Crassostrea sp. | |
40.8 | - |
L-arginine | pH 7.9, 25°C, mutant enzyme K193R | Crassostrea sp. | |
41.9 | - |
ATP | pH 7.9, 25°C, mutant enzyme N62D | Crassostrea sp. | |
41.9 | - |
L-arginine | pH 7.9, 25°C, mutant enzyme N62D | Crassostrea sp. | |
42.1 | - |
ATP | pH 7.9, 25°C, mutant enzyme N62D/K193R | Crassostrea sp. | |
42.1 | - |
L-arginine | pH 7.9, 25°C, mutant enzyme N62D/K193R | Crassostrea sp. | |
47.5 | - |
ATP | pH 7.9, 25°C, wild-type enzyme | Crassostrea sp. | |
47.5 | - |
L-arginine | pH 7.9, 25°C, wild-type enzyme | Crassostrea sp. |