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Literature summary for 2.7.3.2 extracted from
- Dissecting the key residues crucial for the species-specific thermostability of muscle-type creatine kinase (2010), Int. J. Biol. Macromol., 47, 366-370.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Homo sapiens |
| expressed in Escherichia coli | Danio rerio |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| A189D | Km and kcat values similar to wild-type, T0.5 (°C): 46.2 (wild-type: 48.0°C) | Danio rerio |
| A205S | Km and kcat values similar to wild-type, T0.5 (°C): 46.6 (wild-type: 48.0°C) | Danio rerio |
| A267H | Km and kcat values similar to wild-type, T0.5 (°C): 56.9 (wild-type: 56.9°C) | Homo sapiens |
| A329S | Km and kcat values similar to wild-type, T0.5 (°C): 56.6 (wild-type: 56.9°C) | Homo sapiens |
| C146N | Km and kcat values similar to wild-type, T0.5 (°C): 52.4 (wild-type: 56.9°C) | Homo sapiens |
| D189A | Km values 2-3fold decreased compared to wild-type, kcat 30% decreased compared to wild-type, T0.5 (°C): 57 (wild-type: 56.9°C) | Homo sapiens |
| E185Q | Km (ATP) and (creatine) significantly higher compared to wild-type, kcat value 74% of wild-type, T0.5 (°C): 47.4 (wild-type: 48.0°C) | Danio rerio |
| E46Q | Km values 2-3fold decreased compared to wild-type, kcat 30% decreased compared to wild-type, T0.5 (°C): 50.1 (wild-type: 56.9°C) | Homo sapiens |
| H267A | Km and kcat values similar to wild-type, T0.5 (°C): 47.6 (wild-type: 48.0°C) | Danio rerio |
| K304T | Km and kcat values similar to wild-type, T0.5 (°C): 57.1 (wild-type: 56.9°C) | Homo sapiens |
| K36L | Km and kcat values similar to wild-type, T0.5 (°C): 46.7 (wild-type: 48.0°C) | Danio rerio |
| L36K | Km and kcat values similar to wild-type, T0.5 (°C): 47.3 (wild-type: 56.9°C) | Homo sapiens |
| N146C | Km and kcat values similar to wild-type, T0.5 (°C): 51 (wild-type: 48.0°C) | Danio rerio |
| Q185E | Km values 2-3fold decreased compared to wild-type, kcat 30% decreased compared to wild-type, T0.5 (°C): 56.4 (wild-type: 56.9°C) | Homo sapiens |
| Q46E | Km and kcat values similar to wild-type, T0.5 (°C): 50.9 (wild-type: 48.0°C) | Danio rerio |
| S205A | Km and kcat values similar to wild-type, T0.5 (°C): 58.1 (wild-type: 56.9°C) | Homo sapiens |
| S329A | Km and kcat values similar to wild-type, T0.5 (°C): 48.3 (wild-type: 48.0°C) | Danio rerio |
| T304K | Km and kcat values similar to wild-type, T0.5 (°C): 47.6 (wild-type: 48.0°C) | Danio rerio |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.36 | - |
ATP | 25°C, pH not specified in the publication, mutant T304K | Danio rerio |  |
| 0.42 | - |
ATP | 25°C, pH not specified in the publication, mutant S329A | Danio rerio | |
| 0.45 | - |
ATP | 25°C, pH not specified in the publication, mutant N146C | Danio rerio | |
| 0.49 | - |
ATP | 25°C, pH not specified in the publication, wild-type | Danio rerio | |
| 0.58 | - |
ATP | 25°C, pH not specified in the publication, mutant A205S | Danio rerio | |
| 0.58 | - |
ATP | 25°C, pH not specified in the publication, mutant Q46E | Danio rerio | |
| 0.62 | - |
ATP | 25°C, pH not specified in the publication, mutant H267A | Danio rerio | |
| 0.62 | - |
ATP | 25°C, pH not specified in the publication, wild-type | Homo sapiens | |
| 0.65 | - |
ATP | 25°C, pH not specified in the publication, mutant S205A | Homo sapiens | |
| 0.73 | - |
ATP | 25°C, pH not specified in the publication, mutant A267H | Homo sapiens | |
| 0.74 | - |
ATP | 25°C, pH not specified in the publication, mutant K304T | Homo sapiens | |
| 0.74 | - |
ATP | 25°C, pH not specified in the publication, mutant L36K | Homo sapiens | |
| 0.82 | - |
ATP | 25°C, pH not specified in the publication, mutant A189D | Danio rerio | |
| 0.84 | - |
ATP | 25°C, pH not specified in the publication, mutant D189A | Homo sapiens | |
| 0.85 | - |
ATP | 25°C, pH not specified in the publication, mutant K36L | Danio rerio | |
| 0.91 | - |
ATP | 25°C, pH not specified in the publication, mutant C146N | Homo sapiens | |
| 0.92 | - |
ATP | 25°C, pH not specified in the publication, mutant E185Q | Danio rerio | |
| 1.09 | - |
ATP | 25°C, pH not specified in the publication, mutant E46Q | Homo sapiens | |
| 1.24 | - |
ATP | 25°C, pH not specified in the publication, mutant Q185E | Homo sapiens | |
| 1.38 | - |
ATP | 25°C, pH not specified in the publication, mutant A329S | Homo sapiens | |
| 2 | 3.4 | Creatine | 25°C, pH not specified in the publication, mutant A329S | Homo sapiens | |
| 5.9 | - |
Creatine | 25°C, pH not specified in the publication, wild-type | Homo sapiens | |
| 6.6 | - |
Creatine | 25°C, pH not specified in the publication, mutant S205A | Homo sapiens | |
| 7.1 | - |
Creatine | 25°C, pH not specified in the publication, mutant L36K | Homo sapiens | |
| 8.7 | - |
Creatine | 25°C, pH not specified in the publication, mutant A267H | Homo sapiens | |
| 8.9 | - |
Creatine | 25°C, pH not specified in the publication, mutant K304T | Homo sapiens | |
| 10.5 | - |
Creatine | 25°C, pH not specified in the publication, mutant S329A | Danio rerio | |
| 11 | - |
Creatine | 25°C, pH not specified in the publication, mutant N146C | Danio rerio | |
| 11.1 | - |
Creatine | 25°C, pH not specified in the publication, mutant Q185E | Homo sapiens | |
| 12.9 | - |
Creatine | 25°C, pH not specified in the publication, mutant C146N | Homo sapiens | |
| 14.2 | - |
Creatine | 25°C, pH not specified in the publication, wild-type | Danio rerio | |
| 14.8 | - |
Creatine | 25°C, pH not specified in the publication, mutant E46Q | Homo sapiens | |
| 16.7 | - |
Creatine | 25°C, pH not specified in the publication, mutant H267A | Danio rerio | |
| 17.1 | - |
Creatine | 25°C, pH not specified in the publication, mutant Q46E | Danio rerio | |
| 17.8 | - |
Creatine | 25°C, pH not specified in the publication, mutant T304K | Danio rerio | |
| 20.6 | - |
Creatine | 25°C, pH not specified in the publication, mutant D189A | Homo sapiens | |
| 20.8 | - |
Creatine | 25°C, pH not specified in the publication, mutant A205S | Danio rerio | |
| 21.6 | - |
Creatine | 25°C, pH not specified in the publication, mutant K36L | Danio rerio | |
| 28.5 | - |
Creatine | 25°C, pH not specified in the publication, mutant A189D | Danio rerio | |
| 44.1 | - |
Creatine | 25°C, pH not specified in the publication, mutant E185Q | Danio rerio | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + creatine | Homo sapiens | - |
ADP + creatine phosphate | - |
? | |
| ATP + creatine | Danio rerio | - |
ADP + creatine phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Danio rerio | Q90X19 | - |
- |
| Homo sapiens | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + creatine | - |
Homo sapiens | ADP + creatine phosphate | - |
? | |
| ATP + creatine | - |
Danio rerio | ADP + creatine phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| hMMCK | - |
Homo sapiens |
| muscle-type creatine kinase | - |
Homo sapiens |
| muscle-type creatine kinase | - |
Danio rerio |
| zMMCK | - |
Danio rerio |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 36 | - |
- |
Homo sapiens |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 25 | optimal temperature | Danio rerio |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 48 | - |
muscle-type creatine kinase of Danio rerio is less stable compared to human muscle-type creatine kinase, T0.5: 48°C | Danio rerio |
| 56.9 | - |
muscle-type creatine kinase of Danio rerio is less stable compared to human muscle-type creatine kinase, T0.5: 56.9°C | Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 106 | - |
Creatine | co-substrate: ATP, 25°C, pH not specified in the publication, mutant Q185E | Homo sapiens | |
| 112 | - |
Creatine | co-substrate: ATP, 25°C, pH not specified in the publication, mutant A329S | Homo sapiens | |
| 115 | - |
Creatine | co-substrate: ATP, 25°C, pH not specified in the publication, mutant E46Q | Homo sapiens | |
| 117 | - |
Creatine | co-substrate: ATP, 25°C, pH not specified in the publication, mutant D189A | Homo sapiens | |
| 119 | - |
Creatine | co-substrate: ATP, 25°C, pH not specified in the publication, mutant E185Q | Danio rerio | |
| 122 | - |
Creatine | co-substrate: ATP, 25°C, pH not specified in the publication, mutant K304T | Homo sapiens | |
| 126 | - |
Creatine | co-substrate: ATP, 25°C, pH not specified in the publication, mutant K36L | Danio rerio | |
| 128 | - |
Creatine | co-substrate: ATP, 25°C, pH not specified in the publication, mutant T304K | Danio rerio | |
| 132 | - |
Creatine | co-substrate: ATP, 25°C, pH not specified in the publication, mutant Q46E | Danio rerio | |
| 140 | - |
Creatine | co-substrate: ATP, 25°C, pH not specified in the publication, mutant N146C | Danio rerio | |
| 142 | - |
Creatine | co-substrate: ATP, 25°C, pH not specified in the publication, mutant H267A | Danio rerio | |
| 142 | - |
Creatine | co-substrate: ATP, 25°C, pH not specified in the publication, mutant S329A | Danio rerio | |
| 159 | - |
Creatine | co-substrate: ATP, 25°C, pH not specified in the publication, wild-type | Danio rerio | |
| 163 | - |
Creatine | co-substrate: ATP, 25°C, pH not specified in the publication, mutant A189D | Danio rerio | |
| 170 | - |
Creatine | co-substrate: ATP, 25°C, pH not specified in the publication, wild-type | Homo sapiens | |
| 173 | - |
Creatine | co-substrate: ATP, 25°C, pH not specified in the publication, mutant A205S | Danio rerio | |
| 173 | - |
Creatine | co-substrate: ATP, 25°C, pH not specified in the publication, mutant C146N | Homo sapiens | |
| 175 | - |
Creatine | co-substrate: ATP, 25°C, pH not specified in the publication, mutant L36K | Homo sapiens | |
| 178 | - |
Creatine | co-substrate: ATP, 25°C, pH not specified in the publication, mutant S205A | Homo sapiens | |
| 196 | - |
Creatine | co-substrate: ATP, 25°C, pH not specified in the publication, mutant A267H | Homo sapiens | |
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