Protein Variants | Comment | Organism |
---|---|---|
A76G | mutation in the intra-subunit domain-domain interface, similar to wild-type in kinetics and thermal inactivation | Oryctolagus cuniculus |
C74A | mutation in the intra-subunit domain-domain interface, no significant effect on activity and structure, decrease in stability and reactivation | Oryctolagus cuniculus |
C74L | mutation in the intra-subunit domain-domain interface, no significant effect on activity and structure, decrease in stability and reactivation | Oryctolagus cuniculus |
C74M | mutation in the intra-subunit domain-domain interface, no significant effect on activity and structure, decrease in stability and reactivation | Oryctolagus cuniculus |
C74S | mutation in the intra-subunit domain-domain interface, no significant effect on activity and structure, decrease in stability and reactivation | Oryctolagus cuniculus |
G73A | mutation in the intra-subunit domain-domain interface, decrease in activity and stability | Oryctolagus cuniculus |
V72A | mutation in the intra-subunit domain-domain interface, decrease in activity and stability | Oryctolagus cuniculus |
V75A | mutation in the intra-subunit domain-domain interface, no significant effect on activity and structure, decrease in stability and reactivation | Oryctolagus cuniculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.43 | - |
ATP | mutant C74A, 25°C | Oryctolagus cuniculus | |
0.45 | - |
ATP | wild-type, 25°C | Oryctolagus cuniculus | |
0.46 | - |
ATP | mutant A76G, 25°C | Oryctolagus cuniculus | |
0.47 | - |
ATP | mutant C74S, 25°C | Oryctolagus cuniculus | |
0.51 | - |
ATP | mutant V72A, 25°C | Oryctolagus cuniculus | |
0.57 | - |
ATP | mutant V75A, 25°C | Oryctolagus cuniculus | |
0.66 | - |
ATP | mutant C74M, 25°C | Oryctolagus cuniculus | |
0.68 | - |
ATP | mutant G73A, 25°C | Oryctolagus cuniculus | |
0.7 | - |
ATP | mutant C74L, 25°C | Oryctolagus cuniculus | |
8.7 | - |
Creatine | mutant C74A, 25°C | Oryctolagus cuniculus | |
8.9 | - |
Creatine | mutant A76G, 25°C | Oryctolagus cuniculus | |
9 | - |
Creatine | mutant C74S, 25°C | Oryctolagus cuniculus | |
9.1 | - |
Creatine | wild-type, 25°C | Oryctolagus cuniculus | |
13.8 | - |
Creatine | mutant V75A, 25°C | Oryctolagus cuniculus | |
18.3 | - |
Creatine | mutant C74M, 25°C | Oryctolagus cuniculus | |
18.6 | - |
Creatine | mutant C74L, 25°C | Oryctolagus cuniculus | |
21.4 | - |
Creatine | mutant V72A, 25°C | Oryctolagus cuniculus | |
25 | - |
Creatine | mutant G73A, 25°C | Oryctolagus cuniculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryctolagus cuniculus | P00563 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
muscle | - |
Oryctolagus cuniculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
148 | - |
mutant V72A, 25°C | Oryctolagus cuniculus |
256 | - |
mutant C74M, 25°C | Oryctolagus cuniculus |
258 | - |
mutant C74L, 25°C | Oryctolagus cuniculus |
261 | - |
wild-type, 25°C | Oryctolagus cuniculus |
263 | - |
mutant V75A, 25°C | Oryctolagus cuniculus |
266 | - |
mutant C74S, 25°C | Oryctolagus cuniculus |
272 | - |
mutant A76G, 25°C | Oryctolagus cuniculus |
278 | - |
mutant C74A, 25°C | Oryctolagus cuniculus |
8208 | - |
mutant G73A, 25°C | Oryctolagus cuniculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + creatine | - |
Oryctolagus cuniculus | ADP + phosphocreatine | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
mutant G73A, inactivation | Oryctolagus cuniculus |
48 | - |
wild-type, stable up to 48°C, mutant V75A, inactivation | Oryctolagus cuniculus |
58 | - |
wild-type, inactivation | Oryctolagus cuniculus |