Any feedback?
Literature summary for 2.7.3.2 extracted from
- Relating structure to mechanism in creatine kinase (2005), Crit. Rev. Biochem. Mol. Biol., 40, 1-20.
Application
| Application | Comment | Organism |
|---|---|---|
| diagnostics | enzyme is clinically important as an indicator of myocardial and skeletal muscle disorders and for the diagnosis of acute myocardial infarction | Homo sapiens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of soluble monomeric or dimeric mutants | Oryctolagus cuniculus |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| crystal structure analysis of muscle cytosolic isozyme | Bos taurus |
| crystal structure analysis of muscle cytosolic isozyme | Oryctolagus cuniculus |
| crystal structure analysis of muscle-type cytosolic isozyme crystallized with transition state analogue complex | Tetronarce californica |
| crystal structure analysis of muscle-type cytosolic isozyme, and of ubiquitous mitochondrial isozyme, and of cytosolic brain type isozyme | Homo sapiens |
| crystal structure analysis of the cytosolic brain isozyme, and of the cardiac sarcomeric mitochondrial crystallized free or bound to MgATP or transition state analogue complex | Gallus gallus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| formate | mimics the phosphoryl group in the transition state | Bos taurus |  |
| formate | mimics the phosphoryl group in the transition state | Gallus gallus | |
| formate | mimics the phosphoryl group in the transition state | Homo sapiens | |
| formate | mimics the phosphoryl group in the transition state | Oryctolagus cuniculus | |
| formate | mimics the phosphoryl group in the transition state | Tetronarce californica | |
| guanidinium hydrochloride | first dissociation of subunits, then unfolding into random coil | Oryctolagus cuniculus | |
| iodoacetamide | substrates can protect against alkylation | Bos taurus | |
| iodoacetamide | substrates can protect against alkylation | Gallus gallus | |
| iodoacetamide | substrates can protect against alkylation | Homo sapiens | |
| iodoacetamide | substrates can protect against alkylation | Oryctolagus cuniculus | |
| iodoacetamide | substrates can protect against alkylation | Tetronarce californica | |
| nitrate | mimics the phosphoryl group in the transition state | Bos taurus | |
| nitrate | mimics the phosphoryl group in the transition state | Gallus gallus | |
| nitrate | mimics the phosphoryl group in the transition state | Homo sapiens | |
| nitrate | mimics the phosphoryl group in the transition state | Oryctolagus cuniculus | |
| nitrate | mimics the phosphoryl group in the transition state | Tetronarce californica | |
| nitrite | mimics the phosphoryl group in the transition state | Bos taurus | |
| nitrite | mimics the phosphoryl group in the transition state | Gallus gallus | |
| nitrite | mimics the phosphoryl group in the transition state | Homo sapiens | |
| nitrite | mimics the phosphoryl group in the transition state | Oryctolagus cuniculus | |
| nitrite | mimics the phosphoryl group in the transition state | Tetronarce californica | |
| Phenylglyoxal | complete inactivation, reacts on arginine residues | Oryctolagus cuniculus | |
| phosphate | competitive against ATP and phosphocreatine, noncompetitive against ADP and creatine | Homo sapiens | |
| SDS | dissociation of subunits, no unfolding | Oryctolagus cuniculus | |
| sulfate | competitive against ATP and phosphocreatine, noncompetitive against ADP and creatine | Homo sapiens | |
| transition state analogue complex | consists of creatine, MgADP, and planar ions such as nitrate, nitrite, and formate, binding structure | Homo sapiens | |
| transition state analogue complex | creatine, MgADP-, and planar ions such as nitrate, nitrite, and formate | Tetronarce californica |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetic mechanism, the enzyme shows negative cooperativity and nonidentical active sites | Homo sapiens | |
| additional information | - |
additional information | kinetic mechanism, the enzyme shows negative cooperativity and nonidentical active sites | Oryctolagus cuniculus | |
| additional information | - |
additional information | kinetics, the enzyme shows negative cooperativity and nonidentical active sites | Bos taurus | |
| additional information | - |
additional information | kinetics, the enzyme shows negative cooperativity and nonidentical active sites | Tetronarce californica | |
| additional information | - |
additional information | kinetics, the enzyme shows negative cooperativity and nonidentical active sites | Gallus gallus | |
| 0.03 | - |
ADP | pH 7.0, muscle-type cytosolic isozyme | Homo sapiens | |
| 0.04 | - |
ADP | pH 7.0, brain-type cytosolic isozyme | Homo sapiens | |
| 0.11 | - |
ATP | pH 8.0, ubiquitous mitochondrial isozyme | Homo sapiens | |
| 0.13 | - |
ADP | pH 7.0, ubiquitous mitochondrial isozyme | Homo sapiens | |
| 0.15 | - |
ADP | pH 7.0, ubiquitous mitochondrial isozyme | Homo sapiens | |
| 0.51 | - |
phosphocreatine | pH 7.0, brain-type cytosolic isozyme | Homo sapiens | |
| 0.55 | - |
phosphocreatine | pH 7.0, ubiquitous mitochondrial isozyme | Homo sapiens | |
| 0.68 | - |
ATP | pH 8.0, ubiquitous mitochondrial isozyme | Homo sapiens | |
| 0.81 | - |
ATP | pH 9.0, brain-type cytosolic isozyme | Homo sapiens | |
| 0.89 | - |
ATP | pH 9.0, muscle-type cytosolic isozyme | Homo sapiens | |
| 1.01 | - |
Creatine | pH 8.0, ubiquitous mitochondrial isozyme | Homo sapiens | |
| 1.16 | - |
phosphocreatine | pH 7.0, ubiquitous mitochondrial isozyme | Homo sapiens | |
| 1.33 | - |
phosphocreatine | pH 7.0, muscle-type cytosolic isozyme | Homo sapiens | |
| 4.9 | - |
Creatine | pH 9.0, brain-type cytosolic isozyme | Homo sapiens | |
| 7.31 | - |
Creatine | pH 8.0, ubiquitous mitochondrial isozyme | Homo sapiens | |
| 9.5 | - |
Creatine | pH 9.0, muscle-type cytosolic isozyme | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Homo sapiens | 5829 | - |
| cytosol | - |
Bos taurus | 5829 | - |
| cytosol | - |
Oryctolagus cuniculus | 5829 | - |
| cytosol | - |
Tetronarce californica | 5829 | - |
| cytosol | - |
Gallus gallus | 5829 | - |
| mitochondrion | - |
Homo sapiens | 5739 | - |
| mitochondrion | - |
Bos taurus | 5739 | - |
| mitochondrion | - |
Oryctolagus cuniculus | 5739 | - |
| mitochondrion | - |
Tetronarce californica | 5739 | - |
| mitochondrion | - |
Gallus gallus | 5739 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Bos taurus | |
| Mg2+ | - |
Gallus gallus | |
| Mg2+ | binding structure | Homo sapiens | |
| Mg2+ | binding structure | Oryctolagus cuniculus | |
| Mg2+ | binding structure | Tetronarce californica | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 78500 | 85100 | cytosolic muscle isozyme | Homo sapiens |
| 78500 | 85100 | cytosolic muscle isozyme | Bos taurus |
| 78500 | 85100 | cytosolic muscle isozyme | Gallus gallus |
| 82600 | - |
cytosolic muscle isozyme, gel filtration | Oryctolagus cuniculus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + creatine | Homo sapiens | key enzyme in energy homeostasis | ADP + phosphocreatine | - |
r | |
| ATP + creatine | Bos taurus | key enzyme in energy homeostasis | ADP + phosphocreatine | - |
r | |
| ATP + creatine | Oryctolagus cuniculus | key enzyme in energy homeostasis | ADP + phosphocreatine | - |
r | |
| ATP + creatine | Tetronarce californica | key enzyme in energy homeostasis | ADP + phosphocreatine | - |
r | |
| ATP + creatine | Gallus gallus | key enzyme in energy homeostasis | ADP + phosphocreatine | - |
r | |
| additional information | Homo sapiens | probable enzyme evolution, overview | ? | - |
? | |
| additional information | Bos taurus | probable enzyme evolution, overview | ? | - |
? | |
| additional information | Oryctolagus cuniculus | probable enzyme evolution, overview | ? | - |
? | |
| additional information | Tetronarce californica | probable enzyme evolution, overview | ? | - |
? | |
| additional information | Gallus gallus | probable enzyme evolution, overview | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
4 isozymes encoded by 4 different genes: 2 cytosolic isozymes, a muscle-type MM-CK and a brain-type BB-CK or a heterodimer MB-CK, and 2 mitochondrial isozymes, a ubiquitous MiU-CK and a sarcomeric MiS-CK | - |
| Gallus gallus | P11009 | 4 isozymes encoded by 4 different genes: 2 cytosolic isozymes, a muscle-type MM-CK and a brain-type BB-CK or a heterodimer MB-CK, and 2 mitochondrial isozymes, a ubiquitous MiU-CK and a sarcomeric MiS-CK | - |
| Homo sapiens | - |
4 isozymes encoded by 4 different genes: 2 cytosolic isozymes, a muscle-type MM-CK and a brain-type BB-CK or a heterodimer MB-CK, and 2 mitochondrial isozymes, a ubiquitous MiU-CK and a sarcomeric MiS-CK | - |
| Oryctolagus cuniculus | - |
4 isozymes encoded by 4 different genes: 2 cytosolic isozymes, a muscle-type MM-CK and a brain-type BB-CK or a heterodimer MB-CK, and 2 mitochondrial isozymes, a ubiquitous MiU-CK and a sarcomeric MiS-CK | - |
| Tetronarce californica | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant soluble monomeric or dimeric mutant enzymes by affinity chromatography, native enzyme from skeletal muscle, at high ionic strength, low temperature, and by fractionation with ethanol, or by affinity and ion exchange chromatography | Oryctolagus cuniculus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| ATP + creatine = ADP + phosphocreatine | catalytic cysteine, active site residues are Glu226, Glu227, and Asp228, the enzyme follows a random or an ordered bimolecular mechanism dependent on pH, direct phosphoryl transfer, no phosphorylated intermediate, overview | Gallus gallus | |
| ATP + creatine = ADP + phosphocreatine | catalytic cysteine, active site structure involving His66 and Asp326, overview, the enzyme follows a random or an ordered bimolecular mechanism dependent on pH, direct phosphoryl transfer, no phosphorylated intermediate, overview | Tetronarce californica | |
| ATP + creatine = ADP + phosphocreatine | catalytic cysteine, the enzyme follows a random or an ordered bimolecular mechanism dependent on pH, direct phosphoryl transfer, no phosphorylated intermediate, overview | Homo sapiens | |
| ATP + creatine = ADP + phosphocreatine | catalytic cysteine, the enzyme follows a random or an ordered bimolecular mechanism dependent on pH, direct phosphoryl transfer, no phosphorylated intermediate, overview | Bos taurus | |
| ATP + creatine = ADP + phosphocreatine | catalytic cysteine, the enzyme follows a random or an ordered bimolecular mechanism dependent on pH, direct phosphoryl transfer, no phosphorylated intermediate, overview | Oryctolagus cuniculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Gallus gallus | - |
| heart | - |
Gallus gallus | - |
| skeletal muscle | - |
Homo sapiens | - |
| skeletal muscle | - |
Bos taurus | - |
| skeletal muscle | - |
Oryctolagus cuniculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + creatine | - |
Bos taurus | ADP + phosphocreatine | - |
r | |
| ATP + creatine | - |
Oryctolagus cuniculus | ADP + phosphocreatine | - |
r | |
| ATP + creatine | - |
Tetronarce californica | ADP + phosphocreatine | - |
r | |
| ATP + creatine | - |
Gallus gallus | ADP + phosphocreatine | - |
r | |
| ATP + creatine | key enzyme in energy homeostasis | Homo sapiens | ADP + phosphocreatine | - |
r | |
| ATP + creatine | key enzyme in energy homeostasis | Bos taurus | ADP + phosphocreatine | - |
r | |
| ATP + creatine | key enzyme in energy homeostasis | Oryctolagus cuniculus | ADP + phosphocreatine | - |
r | |
| ATP + creatine | key enzyme in energy homeostasis | Tetronarce californica | ADP + phosphocreatine | - |
r | |
| ATP + creatine | key enzyme in energy homeostasis | Gallus gallus | ADP + phosphocreatine | - |
r | |
| ATP + creatine | the reaction equilibrium lies towards ATP production | Homo sapiens | ADP + phosphocreatine | - |
r | |
| ATP + cyclocreatine | i.e. 1-carboxymethy-2-iminoimidazolidine | Oryctolagus cuniculus | ADP + phospho-cyclocreatine | - |
? | |
| ATP + cyclocreatine | i.e. 1-carboxymethyl-2-iminoimidazolidine | Homo sapiens | ADP + phospho-cyclocreatine | - |
? | |
| ATP + glycocyamine | very low activity | Homo sapiens | ADP + glycocyamine phosphate | - |
? | |
| ATP + glycocyamine | very low activity | Oryctolagus cuniculus | ADP + glycocyamine phosphate | - |
? | |
| ATP + N-ethylglycocyamine | - |
Homo sapiens | ADP + N-ethylglycocyamine phosphate | - |
? | |
| ATP + N-ethylglycocyamine | - |
Oryctolagus cuniculus | ADP + N-ethylglycocyamine phosphate | - |
? | |
| additional information | probable enzyme evolution, overview | Homo sapiens | ? | - |
? | |
| additional information | probable enzyme evolution, overview | Bos taurus | ? | - |
? | |
| additional information | probable enzyme evolution, overview | Oryctolagus cuniculus | ? | - |
? | |
| additional information | probable enzyme evolution, overview | Tetronarce californica | ? | - |
? | |
| additional information | probable enzyme evolution, overview | Gallus gallus | ? | - |
? | |
| additional information | substrate binding structure, arginine residues R130, R132, R236, R292, and R320 form a nucleotide phosphate bindig pocket, reaction equilibrium is highly influenced by pH and Mg2+ concentration, substrate specificity of isozymes | Homo sapiens | ? | - |
? | |
| additional information | substrate binding structure, reaction equilibrium is highly influenced by pH and Mg2+ concentration, assay methods, overview, structure-function analysis, substrate specificity of isozymes, the cytosolic isozymes of skeletal muscle shows broad substrate specificity | Oryctolagus cuniculus | ? | - |
? | |
| additional information | substrate binding structure, reaction equilibrium is highly influenced by pH and Mg2+ concentration, substrate specificity of isozymes | Bos taurus | ? | - |
? | |
| additional information | substrate binding structure, reaction equilibrium is highly influenced by pH and Mg2+ concentration, substrate specificity of isozymes | Gallus gallus | ? | - |
? | |
| additional information | substrate binding structure, substrate binding at both subunits | Tetronarce californica | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 40000-44000, mature enzyme | Tetronarce californica |
| dimer | 2 * 40000-44000, mature cytosolic isozymes | Homo sapiens |
| dimer | 2 * 40000-44000, mature cytosolic isozymes | Bos taurus |
| dimer | 2 * 40000-44000, mature cytosolic isozymes | Oryctolagus cuniculus |
| dimer | 2 * 40000-44000, mature cytosolic isozymes | Gallus gallus |
| More | analysis of the structure of cardiac sarcomeric mitochondrial isozyme, free or bound to MgATP or transition state analogue complex | Gallus gallus |
| More | structure-function analysis, the monomeric enzyme is active, the subunits act independently | Oryctolagus cuniculus |
| octamer | 8 * 40000-44000, mature mitochondrial isozymes, can dissociate to dimers dependent on conditions | Homo sapiens |
| octamer | 8 * 40000-44000, mature mitochondrial isozymes, can dissociate to dimers dependent on conditions | Bos taurus |
| octamer | 8 * 40000-44000, mature mitochondrial isozymes, can dissociate to dimers dependent on conditions | Oryctolagus cuniculus |
| octamer | 8 * 40000-44000, mature mitochondrial isozymes, can dissociate to dimers dependent on conditions | Gallus gallus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ATP-creatine transphosphorylase | - |
Homo sapiens |
| ATP-creatine transphosphorylase | - |
Bos taurus |
| ATP-creatine transphosphorylase | - |
Oryctolagus cuniculus |
| ATP-creatine transphosphorylase | - |
Tetronarce californica |
| ATP-creatine transphosphorylase | - |
Gallus gallus |
| CK | - |
Oryctolagus cuniculus |
| More | the enzyme is a member of the phosphagen (guanidino) kinase family | Homo sapiens |
| More | the enzyme is a member of the phosphagen (guanidino) kinase family | Bos taurus |
| More | the enzyme is a member of the phosphagen (guanidino) kinase family | Oryctolagus cuniculus |
| More | the enzyme is a member of the phosphagen (guanidino) kinase family | Tetronarce californica |
| More | the enzyme is a member of the phosphagen (guanidino) kinase family | Gallus gallus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 51.7 | - |
ATP | pH 8.0, ubiquitous mitochondrial isozyme | Homo sapiens | |
| 51.7 | - |
Creatine | pH 8.0, ubiquitous mitochondrial isozyme | Homo sapiens | |
| 75 | - |
ATP | pH 8.0, ubiquitous mitochondrial isozyme | Homo sapiens | |
| 75 | - |
Creatine | pH 8.0, ubiquitous mitochondrial isozyme | Homo sapiens | |
| 78.3 | - |
ADP | pH 7.0, ubiquitous mitochondrial isozyme | Homo sapiens | |
| 78.3 | - |
phosphocreatine | pH 7.0, ubiquitous mitochondrial isozyme | Homo sapiens | |
| 90 | - |
ADP | pH 7.0, ubiquitous mitochondrial isozyme | Homo sapiens | |
| 90 | - |
phosphocreatine | pH 7.0, ubiquitous mitochondrial isozyme | Homo sapiens | |
| 153.5 | - |
ATP | pH 9.0, muscle-type cytosolic isozyme | Homo sapiens | |
| 153.5 | - |
Creatine | pH 9.0, muscle-type cytosolic isozyme | Homo sapiens | |
| 215 | - |
ATP | pH 9.0, brain-type cytosolic isozyme | Homo sapiens | |
| 215 | - |
Creatine | pH 9.0, brain-type cytosolic isozyme | Homo sapiens | |
| 350 | - |
ADP | pH 7.0, brain-type cytosolic isozyme | Homo sapiens | |
| 350 | - |
phosphocreatine | pH 7.0, brain-type cytosolic isozyme | Homo sapiens | |
| 483.3 | - |
ADP | pH 7.0, muscle-type cytosolic isozyme | Homo sapiens | |
| 483.3 | - |
phosphocreatine | pH 7.0, muscle-type cytosolic isozyme | Homo sapiens | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | assay at | Homo sapiens |
| 7 | 9 | assay at | Oryctolagus cuniculus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ADP | - |
Homo sapiens | |
| ADP | - |
Bos taurus | |
| ADP | - |
Oryctolagus cuniculus | |
| ADP | - |
Gallus gallus | |
| ADP | binding structure | Tetronarce californica | |
| ATP | - |
Homo sapiens | |
| ATP | - |
Bos taurus | |
| ATP | - |
Oryctolagus cuniculus | |
| ATP | - |
Gallus gallus | |
| ATP | binding structure | Tetronarce californica | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Homo sapiens | the mitochondrial isozymes, a ubiquitous MiU-CK and a sarcomeric MiS-CK differ in their pI and ar therefore also termed the acidic and basic isozymes | - |
additional information |
| Bos taurus | the mitochondrial isozymes, a ubiquitous MiU-CK and a sarcomeric MiS-CK differ in their pI and ar therefore also termed the acidic and basic isozymes | - |
additional information |
| Gallus gallus | the mitochondrial isozymes, a ubiquitous MiU-CK and a sarcomeric MiS-CK differ in their pI and ar therefore also termed the acidic and basic isozymes | - |
additional information |
| Oryctolagus cuniculus | the mitochondrial isozymes, a ubiquitous MiU-CK and a sarcomeric MiS-CK differ in their pI and are therefore also termed the acidic and basic isozymes | - |
additional information |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
