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Literature summary for 2.7.3.1 extracted from

  • Mizuta, C.; Tanaka, K.; Suzuki, T.
    Isolation, characterization, and cDNA-derived amino acid sequence of glycocyamine kinase from the tropical marine worm Namalycastis sp (2005), Comp. Biochem. Physiol. B, 140, 387-393.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli as maltose-binding protein fusion proteins Namalycastis sp.

General Stability

General Stability Organism
the recombinant GK alpha enzyme is quite unstable, storage on ice for 4 h caused 10% loss of activity, in contrast GK beta enzyme is stable, the instability of GK alpha is most likely due to its expression as a maltose-binding protein fusion protein Namalycastis sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.89
-
ATP native GK Namalycastis sp.
1.02
-
ATP recombinant GK beta Namalycastis sp.
3.38
-
glycocyamine recombinant GK beta Namalycastis sp.
4.7
-
glycocyamine native GK Namalycastis sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Namalycastis sp. 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ essential for activation Namalycastis sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
SDS-PAGE Namalycastis sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + guanidinoacetate Namalycastis sp.
-
ADP + phosphoguanidinoacetate
-
r

Organism

Organism UniProt Comment Textmining
Namalycastis sp. Q6AW42 beta chain
-
Namalycastis sp. Q6AW43 alpha chain
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate fractionation, gel filtration on Sephacryl S-200n, and DEAE-5PW ion exchange chromatography Namalycastis sp.

Source Tissue

Source Tissue Comment Organism Textmining
body wall muscle
-
Namalycastis sp.
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + creatine 0.5% of the activity with glycocyamine Namalycastis sp. ADP + phosphocreatine
-
r
ATP + glycocyamine
-
Namalycastis sp. ADP + phosphoglycocyamine
-
r
ATP + guanidinoacetate
-
Namalycastis sp. ADP + phosphoguanidinoacetate
-
r

Subunits

Subunits Comment Organism
dimer alphabeta, 2 * ca. 40000, SDS-PAGE Namalycastis sp.

Synonyms

Synonyms Comment Organism
GK
-
Namalycastis sp.
glycocyamine kinase
-
Namalycastis sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
24
-
glycocyamine recombinant GK beta Namalycastis sp.
25.3
-
glycocyamine native GK Namalycastis sp.