Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as a His-tagged fusion protein | Corynebacterium glutamicum |
Protein Variants | Comment | Organism |
---|---|---|
DELTA2-15 | Km (N-acetyl-L-glutamate) increased compared to wild-type, kcat decreased compared to wild-type, IC50 (L-arginine) 71fold increased compared to wild-type | Corynebacterium glutamicum |
DELTA2-19 | Km (N-acetyl-L-glutamate) increased compared to wild-type, kcat decreased compared to wild-type, IC50 (L-arginine) 148fold increased compared to wild-type | Corynebacterium glutamicum |
DELTA2-29 | Km (N-acetyl-L-glutamate) increased compared to wild-type, kcat decreased compared to wild-type, IC50 (L-arginine) 163fold increased compared to wild-type | Corynebacterium glutamicum |
DELTA25 | Km (N-acetyl-L-glutamate) highly increased compared to wild-type, kcat highly decreased compared to wild-type, IC50 (L-arginine) 17fold increased compared to wild-type | Corynebacterium glutamicum |
DELTA8 | Km (N-acetyl-L-glutamate) increased compared to wild-type, kcat highly decreased compared to wild-type, IC50 (L-arginine) 16fold increased compared to wild-type | Corynebacterium glutamicum |
E19A | Km (N-acetyl-L-glutamate) similar to wild-type, kcat similar to wild-type, IC50 (L-arginine) 40fold increased compared to wild-type | Corynebacterium glutamicum |
E19R | Km (N-acetyl-L-glutamate) similar to wild-type, kcat similar to wild-type, IC50 (L-arginine) 61fold increased compared to wild-type | Corynebacterium glutamicum |
E281A | Km (N-acetyl-L-glutamate) highly increased compared to wild-type, kcat similar to wild-type, IC50 (L-arginine) 21fold increased compared to wild-type | Corynebacterium glutamicum |
G287A | Km (N-acetyl-L-glutamate) similar to wild-type, kcat decreased compared to wild-type, IC50 (L-arginine) 37fold increased compared to wild-type | Corynebacterium glutamicum |
G287D | Km (N-acetyl-L-glutamate) similar to wild-type, kcat similar to wild-type, IC50 (L-arginine) 40fold increased compared to wild-type | Corynebacterium glutamicum |
H268A | Km (N-acetyl-L-glutamate) similar to wild-type, kcat similar to wild-type, IC50 (L-arginine) 39fold increased compared to wild-type | Corynebacterium glutamicum |
H268N | Km (N-acetyl-L-glutamate) similar to wild-type, kcat similar to wild-type, IC50 (L-arginine) 58fold increased compared to wild-type | Corynebacterium glutamicum |
H26A | Km (N-acetyl-L-glutamate) similar to wild-type, kcat similar to wild-type, IC50 (L-arginine) 54fold increased compared to wild-type | Corynebacterium glutamicum |
R209A | Km (N-acetyl-L-glutamate) similar to wild-type, kcat similar to wild-type, IC50 (L-arginine) 46fold increased compared to wild-type | Corynebacterium glutamicum |
R209K | Km (N-acetyl-L-glutamate) similar to wild-type, kcat similar to wild-type, IC50 (L-arginine) 37fold increased compared to wild-type | Corynebacterium glutamicum |
W23A | Km (N-acetyl-L-glutamate) similar to wild-type, kcat similar to wild-type, IC50 (L-arginine) 4.5fold increased compared to wild-type | Corynebacterium glutamicum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-arginine | - |
Corynebacterium glutamicum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.14 | - |
N-acetyl-L-glutamate | mutant H26A, pH and temperature not specified in the publication | Corynebacterium glutamicum | |
3.25 | - |
N-acetyl-L-glutamate | mutant H268N, pH and temperature not specified in the publication | Corynebacterium glutamicum | |
3.32 | - |
N-acetyl-L-glutamate | mutant G287D, pH and temperature not specified in the publication | Corynebacterium glutamicum | |
3.43 | - |
N-acetyl-L-glutamate | mutant E19R, pH and temperature not specified in the publication | Corynebacterium glutamicum | |
3.52 | - |
N-acetyl-L-glutamate | wild-type, pH and temperature not specified in the publication | Corynebacterium glutamicum | |
3.61 | - |
N-acetyl-L-glutamate | mutant E19A, pH and temperature not specified in the publication | Corynebacterium glutamicum | |
3.72 | - |
N-acetyl-L-glutamate | mutant R209A, pH and temperature not specified in the publication | Corynebacterium glutamicum | |
3.76 | - |
N-acetyl-L-glutamate | mutant H268A, pH and temperature not specified in the publication | Corynebacterium glutamicum | |
3.86 | - |
N-acetyl-L-glutamate | mutant G287A, pH and temperature not specified in the publication | Corynebacterium glutamicum | |
3.89 | - |
N-acetyl-L-glutamate | mutant W23A, pH and temperature not specified in the publication | Corynebacterium glutamicum | |
3.96 | - |
N-acetyl-L-glutamate | mutant R209K, pH and temperature not specified in the publication | Corynebacterium glutamicum | |
6.98 | - |
N-acetyl-L-glutamate | mutant DELTA8, pH and temperature not specified in the publication | Corynebacterium glutamicum | |
7.84 | - |
N-acetyl-L-glutamate | mutant DELTA2-15, pH and temperature not specified in the publication | Corynebacterium glutamicum | |
8.23 | - |
N-acetyl-L-glutamate | mutant DELTA2-19, pH and temperature not specified in the publication | Corynebacterium glutamicum | |
8.45 | - |
N-acetyl-L-glutamate | mutant DELTA2-29, pH and temperature not specified in the publication | Corynebacterium glutamicum | |
13.68 | - |
N-acetyl-L-glutamate | mutant E281A, pH and temperature not specified in the publication | Corynebacterium glutamicum | |
25.74 | - |
N-acetyl-L-glutamate | mutant DELTA25, pH and temperature not specified in the publication | Corynebacterium glutamicum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
SDS-PAGE | Corynebacterium glutamicum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corynebacterium glutamicum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using Ni-NTA chromatography | Corynebacterium glutamicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + N-acetyl-L-glutamate | - |
Corynebacterium glutamicum | ADP + N-acetyl-L-glutamate 5-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
argB | - |
Corynebacterium glutamicum |
NagK | - |
Corynebacterium glutamicum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | - |
N-acetyl-L-glutamate | mutant DELTA25, pH and temperature not specified in the publication, with 1 mM L-arginine | Corynebacterium glutamicum | |
3.2 | - |
N-acetyl-L-glutamate | mutant DELTA25, pH and temperature not specified in the publication | Corynebacterium glutamicum | |
4.2 | - |
N-acetyl-L-glutamate | mutant DELTA8, pH and temperature not specified in the publication, with 1 mM L-arginine | Corynebacterium glutamicum | |
6.4 | - |
N-acetyl-L-glutamate | mutant DELTA8, pH and temperature not specified in the publication | Corynebacterium glutamicum | |
7.2 | - |
N-acetyl-L-glutamate | mutant DELTA2-29, pH and temperature not specified in the publication, with 1 mM L-arginine | Corynebacterium glutamicum | |
7.9 | - |
N-acetyl-L-glutamate | mutant DELTA2-29, pH and temperature not specified in the publication | Corynebacterium glutamicum | |
11.5 | - |
N-acetyl-L-glutamate | mutant DELTA2-19, pH and temperature not specified in the publication, with 1 mM L-arginine | Corynebacterium glutamicum | |
12.4 | - |
N-acetyl-L-glutamate | mutant DELTA2-19, pH and temperature not specified in the publication | Corynebacterium glutamicum | |
13.8 | - |
N-acetyl-L-glutamate | mutant DELTA2-15, pH and temperature not specified in the publication, with 1 mM L-arginine | Corynebacterium glutamicum | |
15.2 | - |
N-acetyl-L-glutamate | mutant DELTA2-15, pH and temperature not specified in the publication | Corynebacterium glutamicum | |
31.4 | - |
N-acetyl-L-glutamate | wild-type, pH and temperature not specified in the publication, with 1 mM L-arginine | Corynebacterium glutamicum | |
32.8 | - |
N-acetyl-L-glutamate | mutant W23A, pH and temperature not specified in the publication, with 1 mM L-arginine | Corynebacterium glutamicum | |
34.9 | - |
N-acetyl-L-glutamate | mutant E281A, pH and temperature not specified in the publication, with 1 mM L-arginine | Corynebacterium glutamicum | |
36.1 | - |
N-acetyl-L-glutamate | mutant G287A, pH and temperature not specified in the publication, with 1 mM L-arginine | Corynebacterium glutamicum | |
36.7 | - |
N-acetyl-L-glutamate | mutant G287A, pH and temperature not specified in the publication | Corynebacterium glutamicum | |
37.4 | - |
N-acetyl-L-glutamate | mutant R209K, pH and temperature not specified in the publication, with 1 mM L-arginine | Corynebacterium glutamicum | |
38.3 | - |
N-acetyl-L-glutamate | mutant G287D, pH and temperature not specified in the publication, with 1 mM L-arginine | Corynebacterium glutamicum | |
38.5 | - |
N-acetyl-L-glutamate | mutant E19A, pH and temperature not specified in the publication, with 1 mM L-arginine | Corynebacterium glutamicum | |
39.7 | - |
N-acetyl-L-glutamate | mutant R209A, pH and temperature not specified in the publication, with 1 mM L-arginine | Corynebacterium glutamicum | |
39.7 | - |
N-acetyl-L-glutamate | mutant W23A, pH and temperature not specified in the publication | Corynebacterium glutamicum | |
41 | - |
N-acetyl-L-glutamate | mutant E281A, pH and temperature not specified in the publication | Corynebacterium glutamicum | |
41.1 | - |
N-acetyl-L-glutamate | mutant H268A, pH and temperature not specified in the publication, with 1 mM L-arginine | Corynebacterium glutamicum | |
41.3 | - |
N-acetyl-L-glutamate | mutant G287D, pH and temperature not specified in the publication | Corynebacterium glutamicum | |
41.9 | - |
N-acetyl-L-glutamate | mutant R209A, pH and temperature not specified in the publication | Corynebacterium glutamicum | |
42.1 | - |
N-acetyl-L-glutamate | mutant E19R, pH and temperature not specified in the publication, with 1 mM L-arginine | Corynebacterium glutamicum | |
42.1 | - |
N-acetyl-L-glutamate | mutant R209K, pH and temperature not specified in the publication | Corynebacterium glutamicum | |
43 | - |
N-acetyl-L-glutamate | mutant H26A, pH and temperature not specified in the publication, with 1 mM L-arginine | Corynebacterium glutamicum | |
43.3 | - |
N-acetyl-L-glutamate | mutant H268N, pH and temperature not specified in the publication | Corynebacterium glutamicum | |
43.4 | - |
N-acetyl-L-glutamate | mutant H26A, pH and temperature not specified in the publication | Corynebacterium glutamicum | |
43.8 | - |
N-acetyl-L-glutamate | mutant H268N, pH and temperature not specified in the publication, with 1 mM L-arginine | Corynebacterium glutamicum | |
44.1 | - |
N-acetyl-L-glutamate | mutant E19A, pH and temperature not specified in the publication | Corynebacterium glutamicum | |
44.5 | - |
N-acetyl-L-glutamate | mutant H268A, pH and temperature not specified in the publication | Corynebacterium glutamicum | |
44.8 | - |
N-acetyl-L-glutamate | mutant E19R, pH and temperature not specified in the publication | Corynebacterium glutamicum | |
45.1 | - |
N-acetyl-L-glutamate | wild-type, pH and temperature not specified in the publication | Corynebacterium glutamicum |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.4 | - |
wild-type, pH and temperature not specified in the publication | Corynebacterium glutamicum | L-arginine | |
1.8 | - |
mutant W23A, pH and temperature not specified in the publication | Corynebacterium glutamicum | L-arginine | |
6.4 | - |
mutant DELTA8, pH and temperature not specified in the publication | Corynebacterium glutamicum | L-arginine | |
6.8 | - |
mutant DELTA25, pH and temperature not specified in the publication | Corynebacterium glutamicum | L-arginine | |
8.6 | - |
mutant E281A, pH and temperature not specified in the publication | Corynebacterium glutamicum | L-arginine | |
14.8 | - |
mutant R209K, pH and temperature not specified in the publication | Corynebacterium glutamicum | L-arginine | |
14.9 | - |
mutant G287A, pH and temperature not specified in the publication | Corynebacterium glutamicum | L-arginine | |
15.7 | - |
mutant H268A, pH and temperature not specified in the publication | Corynebacterium glutamicum | L-arginine | |
16.1 | - |
mutant E19A, pH and temperature not specified in the publication | Corynebacterium glutamicum | L-arginine | |
16.2 | - |
mutant G287D, pH and temperature not specified in the publication | Corynebacterium glutamicum | L-arginine | |
18.5 | - |
mutant R209A, pH and temperature not specified in the publication | Corynebacterium glutamicum | L-arginine | |
21.7 | - |
mutant H26A, pH and temperature not specified in the publication | Corynebacterium glutamicum | L-arginine | |
22.3 | - |
mutant H268N, pH and temperature not specified in the publication | Corynebacterium glutamicum | L-arginine | |
24.4 | - |
mutant E19R, pH and temperature not specified in the publication | Corynebacterium glutamicum | L-arginine | |
28.4 | - |
mutant DELTA2-15, pH and temperature not specified in the publication | Corynebacterium glutamicum | L-arginine | |
59.2 | - |
mutant DELTA2-19, pH and temperature not specified in the publication | Corynebacterium glutamicum | L-arginine | |
65.2 | - |
mutant DELTA2-29, pH and temperature not specified in the publication | Corynebacterium glutamicum | L-arginine |