Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Corynebacterium crenatum |
Protein Variants | Comment | Organism |
---|---|---|
E19R | Km and kcat (N-acetyl-L-glutamate) similar to wild-type, IC50 (L-arginine) approximately 55fold increased compared to wild-type | Corynebacterium crenatum |
H268N | Km and kcat (N-acetyl-L-glutamate) similar to wild-type, IC50 (L-arginine) approximately 55fold increased compared to wild-type | Corynebacterium crenatum |
H268N/H26E | Km and kcat (N-acetyl-L-glutamate) similar to wild-type, IC50 (L-arginine) 875fold increased compared to wild-type | Corynebacterium crenatum |
H268N/H26E/E19R | Km and kcat (N-acetyl-L-glutamate) similar to wild-type, IC50 (L-arginine) 1960fold increased compared to wild-type | Corynebacterium crenatum |
H26E | Km and kcat (N-acetyl-L-glutamate) similar to wild-type, IC50 (L-arginine) approximately 55fold increased compared to wild-type | Corynebacterium crenatum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-arginine | - |
Corynebacterium crenatum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.29 | - |
N-acetyl-L-glutamate | mutant H26E, pH 8.0, 37°C | Corynebacterium crenatum | |
3.33 | - |
N-acetyl-L-glutamate | mutant E19R, pH 8.0, 37°C | Corynebacterium crenatum | |
3.36 | - |
N-acetyl-L-glutamate | mutant H268N/H26E/E19R, pH 8.0, 37°C | Corynebacterium crenatum | |
3.42 | - |
N-acetyl-L-glutamate | wild-type, pH 8.0, 37°C | Corynebacterium crenatum | |
3.42 | - |
N-acetyl-L-glutamate | mutant H268N/H26E, pH 8.0, 37°C | Corynebacterium crenatum | |
3.45 | - |
N-acetyl-L-glutamate | mutant H268N, pH 8.0, 37°C | Corynebacterium crenatum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
recombinant protein | Corynebacterium crenatum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corynebacterium crenatum | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + N-acetyl-L-glutamate | - |
Corynebacterium crenatum | ADP + N-acetyl-L-glutamate 5-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
argB | - |
Corynebacterium crenatum |
NagK | - |
Corynebacterium crenatum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Corynebacterium crenatum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
31.4 | - |
N-acetyl-L-glutamate | wild-type, pH 8.0, 37°C, with 1 mM L-arginine | Corynebacterium crenatum | |
41 | - |
N-acetyl-L-glutamate | mutant H26E, pH 8.0, 37°C, with 1 mM L-arginine | Corynebacterium crenatum | |
41.1 | - |
N-acetyl-L-glutamate | mutant H268N, pH 8.0, 37°C, with 1 mM L-arginine | Corynebacterium crenatum | |
42 | - |
N-acetyl-L-glutamate | mutant E19R, pH 8.0, 37°C, with 1 mM L-arginine | Corynebacterium crenatum | |
44.4 | - |
N-acetyl-L-glutamate | mutant H26E, pH 8.0, 37°C | Corynebacterium crenatum | |
45.1 | - |
N-acetyl-L-glutamate | wild-type, pH 8.0, 37°C | Corynebacterium crenatum | |
45.3 | - |
N-acetyl-L-glutamate | mutant H268N, pH 8.0, 37°C | Corynebacterium crenatum | |
45.6 | - |
N-acetyl-L-glutamate | mutant H268N/H26E, pH 8.0, 37°C, with 1 mM L-arginine | Corynebacterium crenatum | |
45.8 | - |
N-acetyl-L-glutamate | mutant E19R, pH 8.0, 37°C | Corynebacterium crenatum | |
46 | - |
N-acetyl-L-glutamate | mutant H268N/H26E/E19R, pH 8.0, 37°C, with 1 mM L-arginine | Corynebacterium crenatum | |
46.1 | - |
N-acetyl-L-glutamate | mutant H268N/H26E, pH 8.0, 37°C | Corynebacterium crenatum | |
46.5 | - |
N-acetyl-L-glutamate | mutant H268N/H26E/E19R, pH 8.0, 37°C | Corynebacterium crenatum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Corynebacterium crenatum |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.4 | - |
wild-type, pH 8.0, 37°C | Corynebacterium crenatum | L-arginine | |
21 | - |
mutant H26E, pH 8.0, 37°C | Corynebacterium crenatum | L-arginine | |
22 | - |
mutant H268N, pH 8.0, 37°C | Corynebacterium crenatum | L-arginine | |
24 | - |
mutant E19R, pH 8.0, 37°C | Corynebacterium crenatum | L-arginine | |
350 | - |
mutant H268N/H26E, pH 8.0, 37°C | Corynebacterium crenatum | L-arginine | |
784 | - |
mutant H268N/H26E/E19R, pH 8.0, 37°C | Corynebacterium crenatum | L-arginine |
General Information | Comment | Organism |
---|---|---|
malfunction | argB gene coding the N-acetyl-L-glutamate kinase is first overexpressed in the strain SYPA5-5, whereas the L-arginine production is narrowly increased by 15.4% | Corynebacterium crenatum |