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Literature summary for 2.7.2.8 extracted from

  • Shi, D.; Li, Y.; Cabrera-Luque, J.; Jin, Z.; Yu, X.; Zhao, G.; Haskins, N.; Allewell, N.M.; Tuchman, M.
    A novel N-acetylglutamate synthase architecture revealed by the crystal structure of the bifunctional enzyme from Maricaulis maris (2011), PLoS ONE, 6, e28825.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.2.8

Activating Compound

Activating Compound Comment Organism Structure
L-arginine slight activation is observed at 1 mM L-arginine Maricaulis maris

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as a His-tagged fusion protein Maricaulis maris

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structure of Maricaulis maris NAGS/K (mmNAGS/K) at 2.7 A resolution shows that it is a tetramer Maricaulis maris

Protein Variants

Protein Variants Comment Organism
I106M to increase phasing power, three additional amino acids codons are mutated to methionine (I106M, I294M and L367M). Crystals from this mutant protein are diffracted to 2.7 A Maricaulis maris
I294M to increase phasing power, three additional amino acids codons are mutated to methionine (I106M, I294M and L367M). Crystals from this mutant protein are diffracted to 2.7 A Maricaulis maris
L367M to increase phasing power, three additional amino acids codons are mutated to methionine (I106M, I294M and L367M). Crystals from this mutant protein are diffracted to 2.7 A Maricaulis maris

Organism

Organism UniProt Comment Textmining
Maricaulis maris
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using Ni-NTA chromatography Maricaulis maris

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
20.1
-
 pH 7.4, 37°C Maricaulis maris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + N-acetyl-L-glutamate
-
 Maricaulis maris ADP + N-acetyl-L-glutamate 5-phosphate
-
 ?

Subunits

Subunits Comment Organism
tetramer crystal structure Maricaulis maris

Synonyms

Synonyms Comment Organism
NAGS-K enzyme has both N-acetylglutamate synthase and kinases activity Maricaulis maris