Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-Ketoglutarate | only if bound to the PII protein ATP complex | Arabidopsis thaliana | |
PII protein | prevents inhibition by arginine | Arabidopsis thaliana |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-arginine | less inhibitory in the presence of the PII protein | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.74 | - |
ATP | without PII protein | Arabidopsis thaliana | |
2.03 | - |
ATP | in complex with PII protein | Arabidopsis thaliana | |
2.47 | - |
ATP | in complex with PII protein and 2-ketoglutarate | Arabidopsis thaliana | |
7.08 | - |
N-acetyl-L-glutamate | without PII protein | Arabidopsis thaliana | |
7.55 | - |
N-acetyl-L-glutamate | in complex with PII protein | Arabidopsis thaliana | |
9.45 | - |
N-acetyl-L-glutamate | in complex with PII protein and 2-ketoglutarate | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Arabidopsis thaliana | 9507 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
mass spectrometry | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9SCL7 | - |
- |
Purification (Comment) | Organism |
---|---|
affinity chromatography and gel filtration by binding to the PII protein, Ni-nitrilotriacetic acid agarose to purify the recombinant protein | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + N-acetyl-L-glutamate | - |
Arabidopsis thaliana | ADP + N-acetyl-L-glutamate 5-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
acetylglutamate kinase | - |
Arabidopsis thaliana |
N-acetylglutamate kinase | - |
Arabidopsis thaliana |
NagK | - |
Arabidopsis thaliana |
NAGS-K | - |
Arabidopsis thaliana |