Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-arginine | pH and temperature dependent, sigmoidal dependence on concentration of arginine, Hill coefficient of 4, 1 mM, 37°C, 95% inhibition | Thermotoga maritima | |
L-arginine methyl ester | 1 mM, 80% inhibition | Thermotoga maritima | |
additional information | not inhibitory: D-arginine, agmatine, citrulline, L-canavanine, L-lysine, L-ornithine, guanidinium ions, urea at 1 mM, 37°C | Thermotoga maritima |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30341 | - |
6 * 30341, MALDI-TOF MS, 6 * 30344, calculated | Thermotoga maritima |
30344 | - |
6 * 30341, MALDI-TOF MS, 6 * 30344, calculated | Thermotoga maritima |
180000 | - |
gel filtration | Pseudomonas aeruginosa |
180000 | - |
gel filtration | Thermotoga maritima |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
recombinant enzyme | - |
Thermotoga maritima | - |
recombinant enzyme | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Thermotoga maritima |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
45 | - |
37°C, pH 7.5 | Thermotoga maritima |
130 | - |
37°C, pH 7.5 | Pseudomonas aeruginosa |
677 | - |
80°C, pH 7.5 | Thermotoga maritima |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + N-acetyl-L-glutamate | - |
Pseudomonas aeruginosa | ADP + N-acetyl-L-glutamate 5-phosphate | - |
? | |
ATP + N-acetyl-L-glutamate | - |
Thermotoga maritima | ADP + N-acetyl-L-glutamate 5-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 30341, MALDI-TOF MS, 6 * 30344, calculated | Thermotoga maritima |
Synonyms | Comment | Organism |
---|---|---|
NagK | - |
Pseudomonas aeruginosa |
NagK | - |
Thermotoga maritima |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
- |
Thermotoga maritima |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
30 min, complete inactivation | Pseudomonas aeruginosa |
70 | - |
1 h, 100% residual activity | Thermotoga maritima |
80 | - |
1 h, 80% residual activity | Thermotoga maritima |
85 | - |
1 h, 60% residual activity | Thermotoga maritima |